摘要

        通过构建不同循环运行条件和水力停留时间的微生物燃料电池人工湿地（CW MFC），评价其对药物和个人护理用品（PPCP）（磺胺嘧啶（SDZ）、卡马西平（CBZ））的去除和积累能力，萘普生（NPX）和布洛芬（IBP））在四个月的运行过程中。研究了相应的磺胺类抗生素耐药基因（ARGs）的丰度水平。结果表明，CW-MFC的闭路运行有助于降低废水中COD、NH4~+N、PPCPs的质量负荷和废水毒性。此外，低HRT的闭路操作有助于通过电吸附增强选择性PPCP在电极上的质量积累，从而在电极和废水中检测到较高的磺胺ARG丰度。冗余分析结果表明，PPCPs的大量积累对细菌组成有很大影响。Procrustes分析结果进一步表明，细菌群落对ARGs谱有很大贡献。因此，通过网络分析发现ARGs及其寄主菌部分沉积在电极基底上，从而有效地在电极上积累ARGs。通过对毕赤霉预测基因组中细菌群落的功能分析，发现闭路模式提高了代谢功能基因和多个精氨酸的丰度，表明闭路操作对CW-MFC系统的代谢过程和精氨酸积累有积极的影响。

        Microbial fuel cell constructed wetlands (CW-MFCs) with different circuit operation conditions and hydraulic retention time (HRT) were constructed to evaluate their ability to remove and accumulate pharmaceutical and personal care products (PPCPs) (sulfadiazine (SDZ), carbamazepine (CBZ), naproxen (NPX) and ibuprofen (IBP)) during four months running process. The abundance level of corresponding sulfonamide antibiotic resistance genes (ARGs) was also investigated. The results showed that closed circuit operation of CW-MFC contributed to the decrease in mass loading of COD, NH₄⁺-N, PPCPs, and wastewater toxicity in the effluent. Additionally, closed circuit operation with low HRT contributed to enhancing selected PPCP mass accumulation on electrodes by electro-adsorption, and thus the higher sulfonamide ARG abundance was detected in the electrodes and effluent. Moreover, the composition of bacteria was greatly influenced by the mass accumulation of PPCPs revealed by redundancy analysis results. Procrustes analysis results further demonstrated that bacterial community contributed greatly to the ARGs profiles. Therefore, ARGs with their host bacteria revealed by network analysis were partially deposited on electrode substrates, and thus ARGs were effectively accumulated on electrodes. Function analysis of the bacterial community from PICRUSt predicted metagenomes revealed that closed circuit mode enhanced the abundances of the function genes of metabolic and the multiple ARGs, suggesting that closed circuit operation exhibited positive effects on metabolic process and ARG accumulation in CW-MFC system.

        https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(20)30207-1