摘要

       废水通常含有抗生素抗性细菌（ARB）和基因（ARG），它们在废水处理过程中被不同程度地去除。本研究探讨了在亚利桑那州南部两个水资源回收设施中，红霉素核糖体甲基化酶F（erm（F））和1类整合子整合酶（intI1）基因在各阶段的去除情况。尽管Bardenpho处理后基因显著减少，但12个二级出水样本中仍有9个和7个样本观察到erm（F）和intI1。通过沉淀或溶解气浮以及氯消毒的主要工艺对erm（F）和intI1浓度没有显著影响。因此，Bardenpho治疗对降低erm（F）和intI1至关重要。比较了erm（F）和intI1的浓度以及其他人为污染标志物的浓度。本研究结果支持intI1作为检测废水中红霉素抗性基因的合适标记物，因为intI1在高浓度下，在整个处理过程中持续时间更长，并且几乎在每个处理阶段都与erm（F）相关。实践者观点：在废水处理过程中，Bardenpho处理是导致intI1和erm（F）基因减少的关键过程。一级处理和氯消毒对erm（F）和intI1基因浓度没有影响。intI1基因是测定废水中erm（F）基因的合适标记。

        Wastewaters routinely contain antibiotic-resistant bacteria (ARB) and genes (ARG) that are removed to a varying degree during wastewater treatment. This study investigated the removal of the erythromycin ribosome methylase class F (erm(F)) and class 1 integron-integrase (intI1) genes at each stage from two water resource recovery facilities in southern Arizona. Although genes were significantly reduced by Bardenpho treatment, erm(F) and intI1 were still observed in ≥ 9 and 7 out of 12 secondary effluent samples. Primary processes via sedimentation or dissolved air flotation, as well as chlorine disinfection, did not significantly impact erm(F) and intI1 concentrations. Therefore, Bardenpho treatment was critical to reduce erm(F) and intI1. Concentrations of erm(F) and intI1 were compared with each other and other markers for anthropogenic pollution. Results from this study support intI1 as one suitable marker to measure erythromycin resistance genes in wastewater, as intI1 was found at higher concentrations, persisted more throughout treatment, and correlated with erm(F) at nearly every treatment stage. PRACTITIONER POINTS: Bardenpho treatment was the key process responsible for the reduction of intI1 and erm(F) genes during wastewater treatment. Primary treatment and chlorine disinfection did not impact erm(F) and intI1 gene concentrations. The intI1 gene is a suitable marker for measuring erm(F) genes in wastewater.

         https://onlinelibrary.wiley.com/doi/abs/10.1002/wer.1299