摘要

       从动物及其环境中传播的抗生素耐药性是一个对人类健康构成重大威胁的公共问题。本文研究了15份对虾肠道及相关养殖环境（水体和底泥）样品中抗生素抗性基因（ARGs）的多样性和丰度。在虾肠道、池塘水和沉积物样品中分别检测到60个、102个和67个主要属于13个、15个和15个不同类型的arg。射流泵和靶区改性是所有样品的主要阻力机制。结果表明，气单胞菌、耶尔森菌和梭状芽孢杆菌XlVb与ARGs的分布有显著相关性。此外，ARGs的相对丰度与移动遗传因子（MGEs）水平呈正相关。变异分配分析表明，MGEs比细菌群落及其联合效应对抗生素抗性的影响更为重要，占抗性变异的74.46%。总之，本研究为更好地了解ARG在水产养殖环境中的传播和改善水产生态系统的生态管理提供了全面的信息。

        Antibiotic resistance disseminating from animals and their environments is a public issue that poses significant threats to human health. In the present study, the diversity and abundance of antibiotic resistance genes (ARGs) in 15 samples from the guts and related aquaculture environments (water and sediment) of shrimp were investigated. In total, 60 ARGs, 102 ARGs and 67 ARGs primarily belonging to 13, 15 and 15 different types were detected in the shrimp gut, pond water and sediment samples, respectively. Efflux pump and target modification were the predominant resistance mechanisms in all samples. It was found that Aeromonas, Yersinia and Clostridium XlVb were significantly correlated with the distribution of the ARGs. Besides, the relative abundance of ARGs was positively correlated with the levels of mobile genetic elements (MGEs). Moreover, variation partitioning analysis showed that MGEs, contributing to 74.46% of the resistome variation, played an important role in the affecting of the antibiotic resistome than the bacterial communities and their joint effects. Collectively, this study provides comprehensive information to better understand the ARG dissemination in aquaculture environments and to improve the ecological management of aquatic ecosystems.

         https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(18)30080-3