摘要

        研究了猪粪厌氧消化（AD）过程中添加氯化亚铁（FeCl2）对甲烷生成和抗生素抗性基因（ARGs）还原的影响。FeCl2既能提高甲烷累积产量，又能降低总ARGs的丰度，即FC5时CH4产量最大增加21.5%，FC25时ARGs最大减少33.3%。病原菌和金属抗性基因（MRGs）的减少作用增强。通过提高H2利用率和种间直接电子转移（日粮）来提高乙酸和丙酸的利用率，其中FeCl2和乙酸的反应进一步提高了日粮的利用率。细菌群落在ARGs的进化过程中起着重要作用（68.26%），并受到MRGs、移动遗传因子（MGEs）和环境因子的影响。因此，基于FeCl2的AD是提高甲烷产量和降低精氨酸的一种可行而有吸引力的方法。

        In this study, effects of ferrous chloride (FeCl₂) addition on methane production and antibiotic resistance genes (ARGs) reduction were investigated during anaerobic digestion (AD) of swine manure. FeCl₂ could both improve the accumulative methane production and reduce the abundance of total ARGs, i.e., the maximum increase of CH₄ production of 21.5% at FC5, and the maximum ARGs reduction of 33.3% at FC25. The reduction of pathogenic bacteria and metal resistance genes (MRGs) was enhanced. Acetate and propionate utilization were intensified by enhancing H₂ utilization and direct interspecies electron transfer (DIET), where DIET was further enhanced by the reaction of the FeCl₂ and acetic acid. The bacterial community played important role in the evolution of ARGs (68.26%), which were also affected by MRGs, mobile genetic elements (MGEs), and environmental factors. Therefore, FeCl₂-based AD is a feasible and attractive way to improve methane production and ARG reduction.

         https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(19)31749-3