摘要

       采用盆栽试验，模拟不同浓度磺胺甲恶唑污染土壤。采用Illumina高通量测序法对土壤微生物群落结构进行了研究，并用PCR和液滴数字PCR方法对6类抗生素耐药的64个亚型进行了分析。结果表明，土壤磺胺甲恶唑污染120d后对真菌多样性无显著影响（P>0.05），细菌多样性显著降低（P<0.05）。污染土壤微生物群落结构发生显著变化，优势菌属和真菌属与对照土壤差异显著。磺胺甲恶唑污染导致ARG多样性增加，抗磺胺基因sul1的丰度显著增加（P<0.05）。而磺胺类耐药基因sul2、喹诺酮类耐药基因floR和cmlA1、四环素类耐药基因tet（34）、tetG2、tetG1、tetM和tetA/P在污染土壤中的丰度变化不显著（P>0.05）。

        A pot experiment was carried out to simulate soil contaminated by sulfamethoxazole at different concentrations. The community structure of soil microorganisms was investigated using Illumina high-throughput sequencing, and 64 subtypes of antibiotic resistance genes (ARGs) resistant to six classes of antibiotic were also analyzed by PCR and droplet digital PCR. The results showed that soil contamination with sulfamethoxazole had no significant effect on fungal diversity after 120 days (P>0.05) whereas bacterial diversity was significantly reduced (P<0.05). The microbial community structure of the contaminated soil changed significantly, with the dominant bacterial and fungal genera being significantly different from the control soil. Sulfamethoxazole contamination resulted in an increase in ARG diversity, and the abundance of the sulfonamide resistance gene sul1 increased significantly (P<0.05). However, the abundance of the sulfonamide resistance gene sul2, the quinolone resistance genes floR and cmlA1, and the tetracycline resistance genes tet(34), tetG2, tetG1,tetM, and tetA/P did not show significant changes in the contaminated soil (P>0.05).

         https://www.ncbi.nlm.nih.gov/pubmed/31854838