摘要

      鱼类相关的抗生素抗性基因（ARGs）由于其通过食物链对人类的潜在风险而受到越来越多的关注。然而，关于鱼类自身的抗生素耐药性的数据却很少。本文对我国4种主要淡水鲤（鲢、草鱼、鳙、鲫）的肠道抗菌基因（ARGs）进行了研究。结果表明，目标ARGs（tetA、tetO、tetQ、tetW、sulI、sulII和blaTEM-1）和1类整合酶（intI1）的丰度分别为9.4×××10-6-1.6××10-1和6.7××10-5-5.2××10-2个基因拷贝/16srrna。sulI、sulII和tetQ与银和汞抗性基因（如silE和merR）密切相关。基于主成分分析，鱼肠微生物类群在零售市场中可以部分分离。鱼肠中约有15.0%的otu被共享，74.5%的otu被鉴定为酸杆菌、拟杆菌、氯杆菌、蓝藻、厚壁菌和蛋白质细菌。这些门可能构成四种中国淡水鲤内脏的核心微生物群。基于网络分析，揭示了可能的ARG宿主，并且在鱼肠道中存在与病原体相关的抗性属，这突出了充分了解其潜在的人类健康风险的必要性。

       Fish-associated antibiotic resistance genes (ARGs) have attracted increasing attention due to their potential risks to human beings via the food chain. However, data are scarce regarding the antibiotic resistance in fish themselves. Herein, the antibiotic resistance genes (ARGs) were assessed in the gut of four major Chinese freshwater carp (i.e., silver carp, grass carp, bighead carp, and crucian carp) from food retail markets. Results show that the abundances of target ARGs (e.g., tetA, tetO, tetQ, tetW, sulI, sulII, and bla_TEM-1) and class 1 integrase (intI1) were in the range 9.4 × 10^-6 - 1.6 × 10^-1 and 6.7 × 10^-5 - 5.2 × 10^-2 gene copies per 16S rRNA gene, respectively. The sulI, sulII, and tetQ strongly correlated with silver and mercury resistance genes (e.g., silE and merR). The microbial taxa of fish gut could be partly separated among retail markets based on the PCA analysis. About 15.0% of the OTUs in fish gut were shared and 74.5% of the shared OTUs were identified as Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, and Proteobacteria. These phyla may constitute the core microbiota in the guts of the four Chinese freshwater carp. The possible ARG hosts were revealed based on the network analysis, and the presence of pathogen-associated resistant genera in fish gut highlights the need to fully understand their potential human health risks.

        https://www.sciencedirect.com/science/article/pii/S0269749119323942?via%3Dihub