摘要

       研究了两种不同类型养殖场底泥中细胞外和细胞内DNA（eARGs和iARGs）中ARGs的发生和数量。共鉴定出20种ARG亚型与7类常用抗生素（如氨基糖苷类、β-内酰胺类、磺胺类、四环素类）有关，其中一些亚型与所用抗生素无关。ARGs主要以iDNA形式存在，总iARGs与eARGs的比值在7.9-45.5之间。eARGs与iARGs的比值随ARG亚型的不同而变化很大，这可能是由于它们作为eDNA的一部分在持久性上的差异。养殖场底泥中eDNA和iDNA的int1与ARGs呈显著相关。此外，由于牛蛙饲养过程中抗生素的使用频率较高，牛蛙养殖池的ARGs污染较混养池严重。

       The occurrence and quantities of ARGs in extracellular and intracellular DNA (eARGs and iARGs) from sediments collected in two different types of aquaculture farms were investigated. A total of 20 ARG subtypes associated with 7 categories of commonly used antibiotics (e.g., aminoglycosides, beta-lactams, sulfonamides, tetracyclines) were identified, and some of these subtypes were not related to the antibiotics used. ARGs are mainly present in the iDNA form with the ratio of the total iARGs to eARGs being in the range of 7.9-45.5. The ratio of eARG to iARGs varies greatly with ARG subtypes, probably due to their differences in persistence as a part of eDNA. Significant correlation between int1 and ARGs was observed for both eDNA and iDNA in sediments from the aquaculture farms. Moreover, ARG pollution was more serious in bullfrog ponds than polyculture ponds due to the more frequent use of antibiotics in bullfrog rearing operations.

       https://www.sciencedirect.com/science/article/pii/S0045653519313268?via%3Dihub