摘要

       东海沿岸地区自20世纪80年代以来经历了快速的城市化和工业化，造成了严重的环境污染。抗生素耐药基因（ARGs）被认为是一种潜在的高危污染物。以杭州湾（HB）、象山湾（XB）和台州湾（TB）为研究对象，对东海沿岸海域27个ARGs和I类整合子整合酶基因（intI1）的空间分布进行了研究。PCR结果显示，3个海湾沉积物中均存在11个ARGs和intI1。qPCR结果进一步表明，抗磺胺类药物是沉积物中最普遍的ARG类型，抗生素靶点的替换和保护是沉积物中最重要的耐药机制。在ARGs亚型中，sulI、tetW和dfrA13是最丰富的ARGs，其中TB中sulI较高（基于绝对和相对丰度），HB中dfrA13较高（基于相对丰度）。网络分析显示，intI1与tetC、sulI、sulII和blaPSE-1有显著相关性。油是关键的连接因子，与sulI、sulII和blaPSE-1呈显著正相关。此外，重金属、营养盐和有机污染物的共同作用可能是决定沉积物中ARGs命运的关键。

      The coastal area of the East China Sea has experienced rapid urbanization and industrialization in China since 1980s, resulting in severe pollution of its environments. Antibiotic resistance genes (ARGs) are regarded as a kind of emerging pollutant with potential high risk. The sediment samples were collected from Hangzhou Bay (HB), Xiangshan Bay (XB), and Taizhou Bay (TB) to investigate the spatial occurrence and distribution of 27 ARGs and class I integron-integrase gene (intI1) in the coastal area of the East China Sea. The PCR results showed the frequent presence of 11 ARGs and intI1 in the sediments of the three bays. The qPCR results further showed that sulfonamide resistance was the most prevalent ARG type and antibiotic target replacement and protection were the most important resistance mechanisms in the sediments. Regarding the subtype of ARGs, sulI, tetW, and dfrA13 were the most abundant ARGs, in which sulI was higher in TB (based on both the absolute and relative abundances) and dfrA13 was higher in HB (based on the relative abundances). The network analysis revealed that intI1 had significant correlations with tetC, sulI, sulII, and bla_PSE-1. Oil was the key connected factor, which had positive connections with sulI, sulII, and bla_PSE-1. In addition, the joint effect of heavy metals and nutrients & organic pollutants might be crucial for the fate of ARGs in the coastal sediments.

       https://www.sciencedirect.com/science/article/pii/S100107421832895X?via%3Dihub