摘要

      抗生素耐药基因（ARGs）作为一种新兴的环境污染物，近年来引起了人们的广泛关注。目前，抗生素对环境污染的研究主要集中在医药、畜牧业、水产养殖等领域，而在植物保护领域对农业抗生素对环境污染的研究较少。五味子素是一种低毒、高效、广谱的农用抗生素。它在农业生产中得到了广泛的应用，有效地控制了作物真菌病害。本研究在温室条件下，通过盆栽试验，研究了不同土壤处理（A、B、C、D）对典型ARGs和微生物群落命运的影响。实时PCR检测8种典型ARGs，高通量测序分析微生物群落。结果表明，五邑菌素对ARG丰度和绝对基因拷贝数均无显著影响，且各处理间微生物群落差异不显著。由于其只是短期的结果，且ARGs的检测数量有限，因此在长期使用时是否对生态环境安全还需要进一步深入的研究。

       Antibiotic resistance genes (ARGs) have raised numerous concerns in recent years as emerging environmental contaminants. At present, research on environmental contamination by antibiotics focuses on medical, animal husbandry, and aquaculture fields, with few studies on environmental contamination by agricultural antibiotics in the field of plant protection. Wuyiencin is a low toxicity, high efficiency, and broad-spectrum agricultural antibiotic. It has been widely used in agricultural production and it effectively controls crop fungal diseases. In the present study, pot experiments with four soil treatments (A, B, C and D) were set up in a greenhouse to investigate the effect of the application of wuyiencin on the fate of typical ARGs and microbial community. Eight typical ARGs were detected by real-time PCR and the microbial communities were analyzed using high-throughput sequencing. The results showed that wuyiencin neither significantly influenced ARG abundance and absolute gene copy numbers, nor significantly varied microbial community among treatments. Since it only was short-term results, and the detection number of ARGs was limited, whether wuyiencin is safe or not to ecological environment when using for long-term will need further deep research.

        https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408571/