摘要

       用Rappaport-Vassiliadis增菌液和沙门氏菌显色琼脂对650份肉和组织样品进行检测和分离，然后用特异性抗血清和聚合酶链反应（PCR）对其特异性血清型基因组区域（SSGRS）进行确认。采用纸片扩散法检测15种抗生素（CRO、AMX、GEN、STR、TET、CHL、CLR、LVX、OFX、GAT、CIP、SXT、AMP、LIN和AZM），用聚合酶链反应（PCR）检测耐药基因（TET（A）、TET（B）、TET（C）、strA/strB、aadA、aac（3）IV）、aadB、sul1、sul2和sul3、blaCMY-2、blaTEM和blaSHV）。肠道沙门氏菌的总患病率为12%，高于家禽养殖场（37.2%）。所有阳性肉和组织样品的MPN均为3.6mpn/g（0.17-18）。共分离到234株，以血清型（139株）和肠炎（95株）最为常见。不同血清型沙门氏菌的耐药谱不同。总分离株对LIN（93.1%，218/234）高度耐药，其次是AMX（80%，187/234）、AMP（74.3%，174/234）、TET（64.5%，151/234）和STR（64.5%，151/234）。总的来说，最常见的ARG是blaTEM（76%，178/234），其次是blaSHV（71.7%，168/234），tet（A）（64%，151/234）和tet（B）（64%，150/234），而最小的ARG是aadB（7.2%，17/234）。对Balb/C小鼠进行了典型鼠和肠道致病性试验。典型鼠和肠炎均能成功定植，发病率为100%，但肠炎的死亡率为33%。

        For detection and isolation of Salmonella enterica, 650 meat and tissue samples were processed using Rappaport-Vassiliadis Enrichment broth and Salmonella Chromogenic agar followed by confirmation through specific antisera and polymerase chain reaction (PCR) targeting their Specific Serovar Genomic Regions (SSGRS). Isolates were tested for 15 antibiotics (CRO, AMX, GEN, STR, TET, CHL, CLR, LVX, OFX, GAT, CIP, SXT, AMP, LIN and AZM) according to the disc diffusion method and antimicrobial resistant genes (tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), aadB, sul1, sul2 and sul3, blaCMY-2, blaTEM and blaSHV) using PCR. The overall prevalence of Salmonella enterica was 12%, being higher in markets (15%) as compared to poultry farms (37.2%). The MPN of all positive meat and tissue samples was found 3.6 MPN/gram (0.17-18). A total of 234 isolates were obtained, serovar Typimurium (139) and Enteridits (95) were the most prevalent. Antimicrobial resistance patterns were different in different serovars according to origin of Salmonella isolates. The overall isolates were highly resistant for LIN (93.1%, 218/234) followed by AMX (80%, 187/234), AMP (74.3%, 174/234), TET (64.5%, 151/234) and STR (64.5%, 151/234). Overall, the most common ARG was bla_TEM (76%, 178/234), followed by bla_SHV (71.7%, 168/234), tet(A) (64%, 151/234) and tet(B) (64%, 150/234), while the least ARG was aadB (7.2%, 17/234). Both Typimurium and Enteridits were tested in the Balb/C mice for pathogenicity. Both Typimurium and Enteridits were found to cause successful colonization, 100% morbidity but Enteriditis were found to cause 33% mortality.

        https://www.sciencedirect.com/science/article/abs/pii/S0882401018307824?via%3Dihub