摘要

      淡水湖是重要的抗生素耐药基因库。本研究采用高通量定量PCR技术，测定了我国应湖水体中ARG的分布。ARGs在研究区的高流行率表明，ARGs对研究区有明显的污染，其中箱式养鱼区的ARG多样性和丰度高于围栏养鱼区。网络分析表明ARGs与移动遗传因子广泛共存。cphA-01、blalMP02和blaCMY202被认为是估算ARG总量的合适指示基因。冗余分析表明，不同鱼类养殖方式引起的理化参数变化引起的微生物群落变化主要决定了湖泊系统中的ARGs。因此，对影响ARGs的因素进行分析，为研究ARGs在湖泊中的维持和传播机制提供了新的思路。

       Freshwater lakes are important reservoirs for antibiotic resistance genes (ARGs). In this study, we determined the ARG profiles in water samples from Ying Lake, China, using high-throughput quantitative PCR. The high prevalence of ARGs suggested significant pollution with ARGs in the study area, where the ARG diversity and abundance were greater in an area with box-type fish farming than an area with fenced fish farming. Network analysis indicated the widespread co-occurrence of ARGs and mobile genetic elements. cphA-01, blalMP02, and blaCMY202 were identified as adequate indicator genes for estimating the total ARG abundances. Redundancy analysis indicated that changes in the microbial communities caused by variations in the physicochemical parameters with different fish culture methods mainly determined the ARGs in the lake system. Thus, analyzing the factors that affect ARGs provided novel insights into the mechanisms responsible for the maintenance and propagation of ARGs in a lake.

        https://link.springer.com/article/10.1007%2Fs11356-018-3972-0