摘要

      本研究旨在通过添加粘土来降低鸡粪堆肥中抗生素抗性基因（ARGs）的相对丰度，并探讨其作用机理。以PM干重为基础，比较了5种粘土添加剂（0%（T1）、2%（T2）、4%（T3）、6%（T4）、8%（T5）和10%（T6）的用量，探讨了粘土添加剂降低PM堆肥中ARGs的机理。结果表明，在养殖场初始生颗粒物中，ARGs浓度是国内颗粒物的1.7-3.01倍。高剂量的粘土添加剂在降低ARGs含量方面起着重要作用，是导致各处理组ARGs丰度显著差异的主要因素。因此，我们建议添加高剂量的粘土（HDC）作为降低PM堆肥中ARGs最大含量的有效手段。热图相关研究证实，在堆肥过程中添加HDC降低了来自鸡肉饲料的有毒金属的生物可利用部分，并显著影响ARG的分散。

       The aim of the study was to reduce relative abundance of antibiotic resistant genes (ARGs) in the poultry manure (PM) compost by adding clay and to determine the mechanism of this effect. Five doses of clay additive [at 0% (T1), 2% (T2), 4% (T3), 6% (T4), 8% (T5) and 10% (T6) based on PM dry weight] were compared to explore the mechanism of reduction in ARGs in the PM compost by the addition of clay. The results confirmed that in the initial raw PM from the breeding farm, the ARG concentrations were 1.7-3.01 times higher than that in the domestic PM. High doses of the clay additive play an important role in reduction in the ARGs and are the main factor responsible for significant variations in ARG abundance between the treatment groups. Therefore, we recommend adding high doses of clay (HDC) as an effective means to reduce the maximum percentage of ARGs in PM compost. A heat map correlation study confirmed that HDC addition during the composting process reduced the bioavailable fractions of toxic metals originating from the chicken feed and significantly impacted ARG dispersion.

       https://www.sciencedirect.com/science/article/pii/S0045653518324317?via%3Dihub