摘要

      释放到环境中的重金属、药物和其他废物可显著影响环境抗生素耐药性。研究了太湖7个点表层沉积物中22个抗微生物基因（ARGs）和10个重金属含量的变化及其与重金属的关系。结果表明，沉积物中ARG含量与湖泊中特定重金属（Fe、Mn、Cr、Cu、Zn等）存在显著的相关性（p<0.05），特别是四环素和磺胺类（tet（A）、tet（D）、tet（E）、tet（O）、sul1、sul2和int-1）的含量与湖泊中特定重金属（Fe、Mn、Cr、Cu、Zn等）的含量有显著的相关性。表层沉积物中重金属与抗性基因存在交互作用，但交互作用强度随着深度的增加而减弱。对大多数重金属而言，表层沉积物中的元素含量高于其他深度。检测了四环素耐药基因（tet（A）、tet（B）、tet（D）、tet（E）和tet（O）、β-内酰胺耐药基因（SHV、TEM、CTX、OXA和OXY）和磺胺耐药基因（sulA、sul1、sul2、sul3和int-1）。他们显示出一种趋势，即随着深度的增加，这些ARGs的相对丰度（标准化为16srrna基因）在统计学上显著增加，随后减少。研究表明，tet（A）、tet（O）、TEM、OXY、int-1、sul1和sul3在高丰度表层沉积物中广泛存在，表明这些基因值得进一步研究。

       Heavy metals, pharmaceuticals, and other wastes released into the environment can significantly influence environmental antibiotic resistance. We investigated the occurrence of 22 antimicrobial resistance genes (ARGs) and 10 heavy metal concentrations, and the relationship between ARGs and heavy metals in surface sediment from seven sites of Lake Taihu. The results showed significant correlations (p < 0.05) between sediment ARG levels, especially for tetracycline and sulfonamides (e.g., tet(A), tet(D), tet(E), tet(O), sul1, sul2 and int-1) and specific heavy metals (Fe, Mn, Cr, Cu, Zn, among others) in the Lake. In the surface sediments, heavy metals showed an interaction with resistance genes, but the strength of interaction was diminished with increasing depth. For most of the heavy metals, the concentration of elements in the top sediments was higher than that in other depths. Tetracycline resistance genes (tet(A), tet(B), tet(D), tet(E) and tet(O), β-lactam resistance genes (SHV, TEM, CTX, OXA and OXY) and sulfonamide resistance genes (sulA, sul1, sul2, sul3 and int-1) were detected. They showed a trend which inferred a statistically significant increase followed by decreases in the relative abundance of these ARGs (normalized to 16S rRNA genes) with increasing depth. This study revealed that tet(A), tet(O), TEM, OXY, int-1, sul1 and sul3 were widespread in surface sediments with high abundance, indicating that these genes deserve more attention in future work.

        https://www.sciencedirect.com/science/article/pii/S1001074218306867?via%3Dihub