摘要

      畜禽粪便是抗药性基因研究的热点。固体厌氧消化（SAD）是一种高效的畜禽粪便处理方法，但在SAD过程中，其ARGs和微生物群落的变化尚不清楚。因此，本研究采用高温SAD（55℃）、中温SAD（35℃）、液体厌氧消化（35℃）等方法，探讨SAD对ARGs、移动遗传元素和微生物群落的影响。与液体厌氧消化相比，SAD显著降低了至少7/10的ARGs和所有可移动的遗传元素，其中嗜热SAD产物中ARG的总丰度比中温SAD产物高23.7%。硬壁菌和变形菌门是ARGs的主要潜在宿主，它们的丰度降低是SAD过程中ARGs减少的主要原因。本研究表明，SAD是降低畜禽粪便中抗生素抗性基因（ARGs）风险的有效方法。

       Livestock manure is a hotspot for antibiotic resistance genes (ARGs). Solid-state anaerobic digestion (SAD) is a high efficiency livestock manure treatment, but the changes in ARGs and microbial communities during SAD are unknown. Therefore, thermophilic SAD (55 °C), mesophilic SAD (35 °C), and liquid anaerobic digestion (35 °C) were conducted to investigate the impacts of SAD on ARGs, mobile genetic elements, and microbial communities. Compared with liquid anaerobic digestion, SAD significantly reduced at least 7/10 ARGs and all of the mobile genetic elements considered, where the total abundance of ARG was 23.7% higher in the thermophilic SAD product than the mesophilic SAD product. Firmicutes and Proteobacteria were the main potential hosts for ARGs, and their decreased abundances were mainly responsible for the reductions in ARGs during SAD. This study demonstrated that SAD is a promising method for decreasing the ARGs risk in livestock manure.

        https://www.sciencedirect.com/science/article/abs/pii/S0960852418312501?via%3Dihub