摘要

       抗生素耐药基因（ARGs）对各种环境的污染是一个亟待解决的问题，尤其是在重金属污染环境中。研究了高浓度铜猪粪厌氧消化过程中添加氧化石墨烯（GO）对抗生素抗性基因（ARGs）和移动遗传元素（MGEs）的影响。铜胁迫显著增加了ARGs和MGEs的总拷贝数。GO显著降低ARG和MGE拷贝数，其中低GO浓度比高GO浓度表现更好。网络分析表明，大部分ARGs和MGEs共同存在，且它们具有相同的主要潜在宿主菌。通过冗余度分析评价了不同因素对ARG丰度的贡献，发现MGEs对ARGs的命运影响最大。因此，GO主要通过去除MGEs来降低ARGs的丰度。

        The pollution of various environments with antibiotic resistance genes (ARGs) is an urgent problem that needs to be addressed, especially in heavy metal-polluted environments. This study investigated the responses of ARGs and mobile genetic elements (MGEs) to the addition of graphene oxide (GO) to swine manure containing a high concentration copper during anaerobic digestion. The total copy numbers of ARGs and MGEs were significantly enhanced by the pressure due to Cu. GO significantly decreased the ARG and MGE copy numbers, where the low GO concentration performed better than the high GO concentration. Network analysis showed that most of the ARGs and MGEs co-occurred and they shared the same major potential host bacteria. The contributions of different factors to ARG abundances were assessed by redundancy analysis and MGEs had the most important effect on the fate of ARGs. Thus, GO may reduce the abundance of ARGs mainly by removing MGEs.

         https://www.sciencedirect.com/science/article/pii/S0048969718344462?via%3Dihub