摘要

       农业土壤-植物系统中大量的抗药性病原菌（ARPB）和抗药性基因（ARGs）对人类健康和环境安全构成严重威胁。因此，开发有针对性的技术来控制土壤-植物系统中现有的抗药性（AR）污染和潜在的传播是至关重要的。本研究将多价噬菌体（噬菌体）疗法与生物炭改良剂分别应用于土壤-莴苣系统中，以促进ARPB/ARG的消散。联合应用生物炭和多价噬菌体，培养63天后，土壤中大肠杆菌K-12（tetR）和铜绿假单胞菌PAO1（ampR+fosR）及其相应的ARGs（tetM、tetQ、tetW、ampC和fosA）含量显著降低（p<0.05）。联合处理后，莴苣组织中的内生K-12、PAO1和ARGs也得到了类似的结果。此外，高通量测序显示，生物炭和多价噬菌体协同作用提高了土壤和莴苣内生细菌群落的结构多样性和功能稳定性。因此，本研究提出一种新的生物技术，将生物炭修正与多价噬菌体疗法结合起来，以实现ARPB的靶向失活，从而刺激土壤-莴苣系统中的ARG耗散。

       High abundances of antibiotic-resistant pathogenic bacteria (ARPB) and antibiotic resistance genes (ARGs) in agricultural soil-plant systems have become serious threats to human health and environmental safety. Therefore, it is crucial to develop targeted technology to control existing antibiotic resistance (AR) contamination and potential dissemination in soil-plant systems. In this work, polyvalent bacteriophage (phage) therapy and biochar amendment were applied separately and in combination to stimulate ARPB/ARG dissipation in a soil-lettuce system. With combined application of biochar and polyvalent phage, the abundance of Escherichia coli K-12 (tet^R) and Pseudomonas aeruginosa PAO1 (amp^R + fos^R) and their corresponding ARGs (tetM, tetQ, tetW, ampC, and fosA) significantly decreased in the soil after 63 days' incubation (p < 0.05). Similar results for endophytic K-12 and PAO1, and ARGs, were also obtained in lettuce tissues following combined treatment. Additionally, high throughput sequencing revealed that biochar and polyvalent phage synergetically improved the structural diversity and functional stability of the indigenous bacterial communities in soil and the endophytic ones in lettuce. Hence, this work proposes a novel biotechnology that combines biochar amendment and polyvalent phage therapy to achieve targeted inactivation of ARPB, which stimulates ARG dissipation in soil-lettuce systems.

         https://www.sciencedirect.com/science/article/pii/S0269749118307097?via%3Dihub