发布者:抗性基因网 时间:2020-04-16 浏览量:661
摘要
威斯康星州科瓦涅县是一个农业区,以集中饲养动物和施肥农田为主。本研究的目的是对科瓦涅县20个地表水地区的化学和抗生素抗性基因(ARG)进行分析,以更好地了解农业污染与一年内ARG丰度之间的关系。在2016年7月至2017年5月的五个时间点内,从20个地点收集了地表水(n=101)和河床沉积物(n=93)。样本分析了六个基因(erm(B)、tet(W)、sul1、qnrA、intI1和16srRNA)以及水化学和污染指标。地表水中qnrA、intI1和sul1基因显著高于erm(B)和tet(W),但沉积物样品中没有差异。冗余分析表明硝酸盐、大肠杆菌和大肠菌群与沉积物中的tet(W)和intI1基因以及水中的intI1、sul1和tet(W)基因呈正相关。土壤ARG丰度的时间分布特征表明,在科瓦纳县施肥期间,沉积物中ARG丰度的基因丰度显著高于对照组,但地表水的分布特征不明显。这些结果表明,科瓦纳县沉积物是非点源农业污染的聚集场所,并在时间尺度上与施肥有关。
Kewaunee County, Wisconsin is an agricultural area dominated by concentrated animal feeding operations and manure fertilized cropland. The objective of this study was to characterize chemical and antibiotic resistance gene (ARG) profiles of 20 surface water locations in Kewaunee County to better understand relationships between agricultural contamination and ARG abundance over one year. Surface water (n = 101) and bed sediment (n = 93) were collected from 20 sites during five timepoints between July 2016 and May 2017. Samples were analyzed for six genes (erm(B), tet(W), sul1, qnrA, intI1 and 16S rRNA) and water chemistry and pollution indicators. qnrA, intI1 and sul1 genes in surface water were significantly higher than erm(B) and tet(W); however, no difference was present in sediment samples. Redundancy analysis identified positive correlations of nitrate, Escherichia coli, and coliforms with tet(W) and intI1 genes in sediment and intI1, sul1 and tet(W) genes in water. Temporal patterns of ARG abundance were identified with significantly higher gene abundances found in sediment during Kewaunee County's manure fertilization period; however, surface water patterns were not distinct. Together, these results suggest Kewaunee County sediments serve as a site of accumulation for non-point source agricultural pollution and ARGs on a temporal scale associated with manure fertilization.
https://academic.oup.com/femsec/article/94/9/fiy131/5053804