摘要

       以某城市污水处理厂为研究对象，采用A2O-MBR为背景，研究了污泥和水体中抗生素耐药基因（ARGs）的差异命运。研究了微生物群落、重金属共选择、生物量和水平基因转移对ARGs的影响。结果表明，iA2O（倒置A2O）对ARGs有较好的还原作用，污泥停留时间越长，ARGs相对丰度越高，而ARGs基因拷贝数越少，但污泥相ARGs明显增加。与本底值相比，水相中富集程度最高的ARG为tetX，而泥相中富集程度最高的ARG为intI1。污泥期存在着丰富的多重耐药菌群，微生物群落决定了ARGs在水体和污泥期的去向，而水平基因转移的直接影响尤其是在水体中更是不容忽视。

        The discrepant fate of antibiotic resistance genes (ARGs) in sludge and water phases was investigated in a municipal wastewater treatment plant, and a lab-scale A²O-MBR was operated to provide background value of ARGs. The influencing factors of ARGs including microbial community, co-selection from heavy metals, biomass and horizontal gene transfer were concerned. Results showed that iA²O (inversed A²O) showed better ARGs reduction, and longer SRT (sludge retention time) increased ARGs relative abundance while reduced the gene copies of ARGs in the effluent, but significantly increased the ARGs in sludge phase. Compared to background value, the most enriched ARG was tetX in water phase, while it was intI1 in sludge phase. There existed higher abundance of multi-resistant bacteria in sludge phase, and microbial community determined the fate of ARGs in both water and sludge phase, while the direct effects from horizontal gene transfer should not be overlooked especially in water phase.

        https://www.sciencedirect.com/science/article/abs/pii/S0960852418308009?via%3Dihub