摘要

       抗生素的控制是防止抗生素耐药基因（ARGs）传播的关键步骤，紫外光催化已被认为是一种很有前途的抗生素去除前处理技术。然而，有关抗生素废水处理后中间产物对下游生物处理工艺或精氨酸（ARGs）开发的影响的信息非常有限。在本研究中，连续UVA/LED/TiO2光催化去除100 ppb磺胺甲恶唑（SMX）/甲氧苄啶（TMP）的90%以上，将处理后的废水送入SBR系统进行一年多的监测。处理后的废水中残留的SMX/TMP（2-3 ppb）和中间产物不会对SBR去除TOC和TN的性能产生不利影响。在SBRs微生物群中，SMX和TMP抗性基因（sulI、sulII、sulII、dfrII、dfrV和dfr13）也被定量。结果表明，连续投加处理后的含SMX/TMP废水，在一年的运行期内不会引起任何ARGs的升高。通过停止100 ppb-SMX/TMP的输入，sulII和dfrV基因的丰度分别降低了83%和100%。sulI基因被认为是最持久的ARG，而控制100 ppb-SMX输入并不能显著去除sulI基因。sulI基因与1类整合子在p = 1.4E-10（r = 0.94）水平上有显著相关性，sulII基因与质粒转移效率呈正相关（r = 2.442E-10，r = 0.87）。连续输入100 ppb-SMX可提高SBR系统中质粒的转移效率，使sulII基因丰度增加40倍以上。

        Controlling of antibiotics is the crucial step for preventing antibiotic resistance genes (ARGs) dissemination; UV photocatalysis has been identified as a promising pre-treatment technology for antibiotics removal. However, information about the effects of intermediates present in the treated antibiotics wastewater on the downstream biological treatment processes or ARGs development is very limited. In the present study, continuous UVA/LED/TiO₂ photocatalysis removed more than 90% of 100 ppb sulfamethoxazole (SMX)/trimethoprim (TMP), the treated wastewater was fed into SBR systems for over one year monitoring. Residual SMX/TMP (2-3 ppb) and intermediates present in the treated wastewater did not adversely affect SBR performance in terms of TOC and TN removal. SMX and TMP resistance genes (sulI, sulII, sulIII, dfrII, dfrV and dfr13) were also quantified in SBRs microbial consortia. Results suggested that continuous feeding of treated SMX/TMP containing wastewaters did not trigger any ARGs promotion during the one year operation. By stopping the input of 100 ppb SMX/TMP, abundance of sulII and dfrV genes were reduced by 83% and 100%, respectively. sulI gene was identified as the most persistence ARG, and controlling of 100 ppb SMX input did not achieve significant removal of sulI gene. A significant correlation between sulI gene and class 1 integrons was found at the level of p = 1.4E-10 (r = 0.94), and sulII gene positively correlated with the plasmid transfer efficiency (r = 2.442E-10, r = 0.87). Continuous input of 100 ppb SMX enhanced plasmid transfer efficiency in the SBR system, resulting in sulII gene abundance increasing more than 40 times.

       https://www.sciencedirect.com/science/article/abs/pii/S0043135418303476?via%3Dihub