摘要

       苍蝇在抗微生物细菌（ARB）的传播中起着重要的载体作用，并被假设在家畜饲养区内外传播ARB。本研究的目的是了解果蝇在农业环境中维持ARB的作用。我们首先评估了在家蝇（Musca domestica）整个生命周期中，从成虫到下一代F1代，摄入的带有抗微生物基因（ARGs）质粒的抗微生物大肠杆菌的命运。从不同的生命周期阶段分离到耐药大肠杆菌，并对其ARG载体进行了定量分析。摄入的大肠杆菌在苍蝇的整个生命周期中都持续存在，家蝇微生物群中的ARG携带保持在恒定水平。为了阐明ARB在家畜中的传播，30日龄鸡接种了含有抗药性大肠杆菌的蛆。根据从盲肠样本中分离出的细菌的数量，抗药性大肠杆菌在这些鸡体内至少持续了16天。这些结果表明，苍蝇在其整个生命周期中都是ARB的贮存者，因此可能参与了ARB在农场环境中的维持和循环。

       Flies play an important role as vectors in the transmission of antimicrobial-resistant bacteria (ARB) and are hypothesized to transfer ARB between internal and external livestock housing areas. The aim of this study was to understand the role that flies may play in the maintenance of ARB in the farm environment. We first evaluated the fate of ingested antimicrobial-resistant Escherichia coli harboring a plasmid containing antimicrobial-resistance genes (ARGs) throughout the housefly (Musca domestica) life cycle, from adult to the subsequent F1 generation. Antimicrobial-resistant E. coli was isolated from different life cycle stages and ARG carriage quantified. The ingested E. coli persisted throughout the fly life cycle, and ARG carriage was maintained at a constant level in the housefly microbiota. To clarify the transmission of ARB from flies to livestock, 30-day-old chickens were inoculated with maggots containing antimicrobial-resistant E. coli. Based on the quantification of bacteria isolated from cecal samples, antimicrobial-resistant E. coli persisted in these chickens for at least 16 days. These results suggest that flies act as a reservoir of ARB throughout their life cycle and may therefore be involved in the maintenance and circulation of ARB in the farm environment.

       https://www.liebertpub.com/doi/abs/10.1089/mdr.2017.0371?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=mdr