摘要

       研究表明，猪粪是抗生素抗性基因（ARGs）的贮存库。然而，对不同日粮喂养的仔猪和成年猪粪便中抗性基因的特性知之甚少。采用高通量荧光定量PCR技术，对不同日粮（饲料、谷物）饲喂的仔猪和成年猪粪便中抗性基因（ARG）的特性进行了分析。分析了猪粪中重金属、抗生素和抗性基因的相关性。结果表明，饲喂猪粪中重金属和抗生素的含量显著高于饲粮猪粪。仔猪粪便中重金属和抗生素含量均高于成年猪。饲料显著提高了猪粪中抗性基因的多样性。仔猪粪中抗性基因的多样性高于成年猪。在饲喂猪粪中，25头（仔猪）和12头（成年猪）抗性基因较饲喂粮猪显著富集。特别是，sat4（仔猪）和vatE-01（成年猪）表现出最高的富集度，分别增加了59倍和19倍。ARG多样性与肥料中抗生素和重金属含量呈正相关。

       Studies have shown that pig manure is a reservoir of antibiotic resistance genes (ARGs). However, little is known about the characteristics of ARGs in the manure of piglets and adult pigs fed on different diets. In the present study, the ARG characteristics of the manure of piglets and adult pigs fed on different diets (feed, grain) were analyzed using high-throughput fluorescence quantitative PCR. Correlations between heavy metals, antibiotics, and ARGs in pig manure were analyzed. The results showed that the heavy metal and antibiotic contents in the manure of pigs receiving feed significantly exceeded those in the manure of pigs receiving grain. The heavy metal and antibiotic contents were higher in manure of piglets than in that of adult pigs. Feed significantly increased the ARG diversity in the pig manure. The ARG diversity was higher in manure of piglets than in that of adult pigs. In the manure of pigs receiving feed, 25 (from piglets), 12 (from adult pigs) ARGs were enriched significantly compared with pig fed with grain. In particular, sat4 (in piglets) and vatE-01 (in adult pigs) showed the highest enrichment, being increased by 59 and 19-fold, respectively. The ARG diversity correlated positively with the concentrations of antibiotics and heavy metals in the manure.

       https://www.sciencedirect.com/science/article/pii/S0269749117310321?via%3Dihub