摘要

    在水环境中传播的抗生素抗性基因（ARG）引起了全世界的关注。在这里，抗生素抗性细菌（ARB）暴露于天然闪锌矿（NS）的界面中，该界面受到水中不同光源（UV254nm，UV365nm，模拟日光（SS）和可见光（VL））的辐射及其对ARG的影响研究了共轭转移。在暴露于光催化（PC）NS + UV254nm或PCNS + VL下，共轭转移频率增加2到10倍，而在PCNS + UV365nm或PCNS + SS下，共轭转移频率增加1到2倍。在暴露过程中，细菌被光照射或自发的光催化失活直接刺激，从而引发氧化应激，DNA修复，细胞修复和ARB的转移调节。这些结果表明，一旦ARB参与该环境PC接口，就可以促进ARG转移。这一新观点表明，自然光照射下的天然矿物质在ARG的传播中起着重要作用。此外，该研究为PC灭菌和ARG转移控制的实际应用提供了更详细的信息。

    Antibiotic-resistance genes (ARGs) dissemination in water environment has raised concerns worldwide. Here, antibiotic-resistant bacteria (ARB) were exposed to the interface of natural sphalerite (NS) irradiated by different light sources (UV254nm, UV365nm, simulated sunlight (SS), and visible light (VL)) in water and their effects on ARG conjugative transfer were studied. The conjugative transfer frequency increased 2- to 10-fold under exposure to Photocatalysis (PC)NS+UV254nm or PCNS+VL and 1- to 2-fold under PCNS+UV365nm or PCNS+SS. During exposure, bacteria were directly stimulated by light irradiation or spontaneous photocatalytic inactivation, triggering oxidative stress, DNA repair, cell repair and transfer regulation of ARB. These results indicated that ARG transfer could be promoted once ARB involving in this environmental PC interface. This new viewpoint revealed that natural minerals with natural light irradiation play an important role in ARG dissemination. Besides, this study provided more detailed information for practical application of PC sterilization and control of ARG transfer.

    https://www.sciencedirect.com/science/article/abs/pii/S092633732100103X