摘要

    垃圾填埋场是地球上最大的人为垃圾处理和处置库，数十年来，它们一直通过渗滤液不断向环境中释放抗生素抗性基因（ARG）。关于时间的函数，ARG和人类细菌病原体之间的关联知之甚少。在这里，我们量化了ARG，整合素和人类细菌病原体（HBP）的10个亚型。除了ARGs编码对磺酰胺类的抗性外，亚型编码对β-内酰胺类，大环内酯类和氨基糖苷类的抗性与整合素无关（Spearman，P> 0.05）。随着时间的流逝，ARG的存在与人类细菌性病原体的存在之间的相关性越来越高（Procrustes测试； R = 0.81，P <0.05），这些病原体主要被鉴定为变形杆菌，放线菌和Firmicutes。而非整合素的流行，细菌群落的动态，包括变形杆菌氮代谢活性的提高和细菌多样性的降低，被认为会导致HBP与目标ARG之间的关联性增加（Varpart；> 13％）。

    Landfills constitute the largest treatment and disposal reservoirs of anthropogenic waste on earth and they are continuously releasing antibiotic resistance genes (ARGs) to the environment for decades via leachates. Little is known about the association between ARGs and human bacterial pathogens as a function of time. Here, we quantified 10 subtypes of ARGs, integrons, and human bacterial pathogens (HBPs). Except for the ARGs encoding resistance to sulfonamides, the subtypes encoding resistance to beta-lactams, macrolides, and aminoglycosides were not related to integrons (Spearman, P > 0.05). Over time presence of ARGs became increasingly more correlated with the presence of human bacterial pathogens (Procrustes test; R = 0.81, P < 0.05), which were primarily identified as the Proteobacteria, Actinobacteria, and Firmicutes. Rather than the prevalence of integrons, dynamics of the bacterial community, including the increased nitrogen metabolism activity of Proteobacteria and decreased bacterial diversity were assumed to lead to a magnified association between HBPs and target ARGs (Varpart; > 13%).

    https://www.sciencedirect.com/science/article/abs/pii/S0045653520326412