摘要

    抗生素及其相应的抗生素抗性细菌（ARB）和抗生素抗性基因（ARG）的出现给公众健康带来了巨大挑战。本文证明了通过应用高铁酸盐（VI）（FeVIO42−，Fe（VI））在混合系统中去除共存的四环素（TC），其抗性大肠杆菌（E. coli）和ARGs（tetA和tetR）在pH 7.0下。 TC被Fe（VI）有效降解，发现抗性大肠杆菌迅速失活，完全丧失了可培养性。流式细胞仪的结果表明，膜完整性和呼吸活动的损害与Fe（VI）剂量高度相关。此外，高剂量的Fe（VI）消除了6个log10存活但不可培养的（VBNC）细胞，甚至将细胞破碎成碎片。细胞外形式的ARG（e-ARGs）对Fe（VI）表现出4.44 log10去除的高灵敏度。相比之下，由于细胞结构的多重保护和Fe（VI）的快速降解，未观察到细胞内ARGs（i-ARGs）的去除。 TC的氧化产物被评估为毒性低于母体化合物。总的来说，这项研究证明了Fe（VI）在同时去除水中的抗生素及其相关的ARB和ARG方面具有卓越的效率和广阔的前景。

    The emergence of antibiotics and their corresponding antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have posed great challenges to the public health. The paper demonstrates the removal of co-existing tetracycline (TC), its resistant Escherichia coli (E. coli), and ARGs (tetA and tetR) in a mixed system by applying ferrate(VI) (FeVIO42−, Fe(VI)) at pH 7.0. TC was efficiently degraded by Fe(VI), and the rapid inactivation of the resistant E. coli was found with the complete loss of culturability. The results of flow cytometry suggested that the damage of membrane integrity and respiratory activity were highly correlated with the Fe(VI) dosages. Moreover, high-dose Fe(VI) eliminates 6 log10 viable but non-culturable (VBNC) cells and even breaks the cells into fragments. ARGs in extracellular form (e-ARGs) exhibited a high sensitivity of 4.44 log10 removal to Fe(VI). Comparatively, no removal of intracellular ARGs (i-ARGs) was observed due to the multi-protection of cellular structure and rapid decay of Fe(VI). The oxidized products of TC were assessed to be less toxic than the parent compound. Overall, this study demonstrated the superior efficiency and great promise of Fe(VI) on simultaneous removal of antibiotics and their related ARB and ARGs in water.

    https://www.sciencedirect.com/science/article/abs/pii/S0048969721025638