摘要

    抗生素抗性基因（ARG）是环境中正在出现的污染物，并已成为全球范围内对环境和健康的关注。作为生物地球化学循环的重要参与者，红树林生态系统受到各种人为干扰，其微生物群可能会受到各种污染物（例如ARGs）的影响。这项研究选择了中国海南红树林覆盖地区的13个样地进行沉积物样品收集。使用高通量定量聚合酶链反应（HT-qPCR）研究了ARG和移动遗传元件（MGE）的丰度和多样性，并使用高通量测序研究了微生物的结构和多样性。在研究区域共检测到179种属于9种ARG类型的ARG，vanXD和vatE-01的检出率为100％。每克沉积物中ARGs的丰度为8.30×107–6.88×108份（每个16S rRNA基因为1.27×10−2–3.39×10−2份），高于同类研究，并且丰度差异很大。这些采样样点中的ARG。多药耐药基因（MRGs）所占比例最高（69.0％），这表明研究区ARGs的污染非常复杂。 ARGs与MGEs显着正相关，这表明ARGs的高水平与其自我增强有关。在属水平上占优势的细菌是脱球菌，梭菌，杜鹃花，芽孢杆菌，弧菌，肠球菌，Sedimentibacter，假单胞菌，副球菌，颤菌，马里氏菌，磺脲类，氨基杆菌和新孢子菌。 133个细菌属与某些ARG之间存在显着的正相关。嗜盐杆菌，黄杆菌杆菌，福氏杆菌，凯斯蒂亚，莫雷利亚，MSBL3，Perlucidibaca和Zhouia是海南红树林地区沉积物中ARGs的主要潜在宿主，其中许多细菌是生物地球化学循环的重要参与者。研究结果有助于我们了解ARGs的分布和潜在宿主，并为保护和管理海南红树林生态系统提供科学依据。

    Antibiotic resistance genes (ARGs) are emerging contaminants in the environment and have been highlighted as a worldwide environmental and health concern. As important participants in the biogeochemical cycles, mangrove ecosystems are subject to various anthropogenic disturbances, and its microbiota may be affected by various contaminants such as ARGs. This study selected 13 transects of mangrove-covered areas in Hainan, China for sediment sample collection. The abundance and diversity of ARGs and mobile genetic elements (MGEs) were investigated using high-throughput quantitative polymerase chain reaction (HT-qPCR), and high-throughput sequencing was used to study microbial structure and diversity. A total of 179 ARGs belonging to 9 ARG types were detected in the study area, and the detection rates of vanXD and vatE-01 were 100%. The abundance of ARGs was 8.30 × 107–6.88 × 108 copies per g sediment (1.27 × 10−2–3.39 × 10−2 copies per 16S rRNA gene), which was higher than similar studies, and there were differences in the abundance of ARGs in these sampling transects. The multidrug resistance genes (MRGs) accounted for the highest proportion (69.0%), which indicates that the contamination of ARGs in the study area was very complicated. The ARGs significantly positively correlated with MGEs, which showed that the high level of ARGs was related to its self-enhancement. The dominant bacteria at the genus level were Desulfococcus, Clostridium, Rhodoplanes, Bacillus, Vibrio, Enterococcus, Sedimentibacter, Pseudoalteromonas, Paracoccus, Oscillospira, Mariprofundus, Sulfurimonas, Aminobacterium, and Novosphingobium. There was a significant positive correlation between 133 bacterial genera and some ARGs. Chthoniobacter, Flavisolibacter, Formivibrio, Kaistia, Moryella, MSBL3, Perlucidibaca, and Zhouia were the main potential hosts of ARGs in the sediments of Hainan mangrove area, and many of these bacteria are important participants in biogeochemical cycles. The results contribute to our understanding of the distribution and potential hosts of ARGs and provide a scientific basis for the protection and management of Hainan mangrove ecosystem.

    https://www.sciencedirect.com/science/article/abs/pii/S0269749121002839