摘要

    结合宏基因组学和定量聚合酶链反应方法，以评估厌氧消化的原地粪便中ARGs和质粒的分布，并揭示了典型ARGs和质粒在其随后在CAFOs中的处置设施中的持久性和高度。我们的结果表明，典型的ARG和plasimd主要是sul2，mefa，tetm-01，tetm-02，tetw，aph3iiia和难辨梭状芽胞杆菌菌株12038在CAFO中未命名的质粒，其中一些在保存后尤其富含AD残留，特别是sul1和sul2。同时，AD泥浆的回收将携带ARGs的细菌引入土壤，特别是Romboutsia属，这种细菌极大地丰富了sul2，tetm-01，tetm-02，蚜虫和mefa。在本研究中，通过对奶牛粪AD处理和随后在CAFOs中处置的过程进行原位分析，可以了解ARGs的发生，持久性和分布，这有助于控制奶牛粪便回收的潜在环境风险。

    The metagenomic and quantitative polymerase chain reaction approaches were combined to evaluate the profiles of ARGs and plasmids in anaerobically digested dairy manure in situ and reveal the persistence and elevation of typical ARGs and plasmids in its subsequent disposal facilities in CAFOs, respectively. Our results indicated that the typical ARGs and plasimd were mainly sul2, mefa, tetm-01, tetm-02, tetw, aph3iiia, and clostridioides difficile strain 12,038 plasmid unnamed in CAFOs, some of which greatly enriched in AD residue after its storage, especially sul1 and sul2. Meantime, the AD slurry recycling introduced the bacteria carrying ARGs into soil, especially Romboutsia genus, which greatly enriched sul2, tetm-01, tetm-02, aphiiia, and mefa. In the present study, ARGs occurrence, persistence and distribution were understood through in situ analysis of their profiles during dairy manure AD treatment and subsequent disposals in CAFOs, which are helpful for controlling the potential environmental risks from dairy manure recycling.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852421003278