摘要

    氯四环素（CTC）是牛粪中存在的最多的抗生素之一。 在本研究中，向牛粪堆肥系统中添加了三级四氯化碳（0、20和40 mg kg-1），以研究其对抗生素抗性基因（ARGs）分布和细菌群落演替的影响。 添加四氯化碳阻碍了堆肥过程中ARG的去除。 高水平的四氯化碳显着增加了9/11 ARG和四个MGE的相对丰度（RA）。 细菌群落可以根据堆肥时间在各种处理下聚集，高水平的四氯化碳对细菌群落具有更持久的影响。 根据冗余分析，细菌群落解释了ARGs的最大差异（50.1％），而基于网络分析，Firmicutes和Proteobacteria是ARGs的主要宿主。 总之，四氯化碳的存在增加了在堆肥产品中传播ARG的风险。

    Chlorotetracycline (CTC) is one of the most antibiotics present in cattle manure. In present study, three levels of CTC (0, 20 and 40 mg kg−1) were added to cattle manure composting systems to investigate its effects on the distribution of antibiotic-resistant genes (ARGs) and succession of bacterial community. Adding CTC hindered the removal of ARGs during composting; the high level of CTC significantly increased the relative abundance (RA) of 9/11 ARGs and four MGEs. The bacterial community could be clustered according to the composting time under various treatments, with the high level of CTC having a more persistent effect on the bacterial community. Based on redundancy analysis, bacterial community explained the most variation in ARGs (50.1%), whereas based on network analysis, Firmicutes and Proteobacteria were the main hosts for ARGs. In conclusion, the presence of CTC increased the risks of spreading ARGs in compost products.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420317910