摘要

    据报道，医院废水（HWW）是抗生素和抗药性细菌的热点地区。 然而，有限的信息涉及这些废水对抗生素抗性基因（ARGs）的传播的影响。 因此，在这项研究中，通过定量聚合酶链反应法对季节性收集的HWW进行了总体细菌负荷监测，并监测了7个ARGs aadA，tetA，cmlA，sul1，qnrS，ermB和bla CTX-M。 发现细菌的总16S rRNA拷贝数在冬季最低，为103拷贝数/ mL，而在夏季和春季均观察到最高拷贝数，为105拷贝数/ mL。 所有接受测试的医院都显示出类似的aadA> tetA> cmlA≈sul1> ermB≈qnrS> bla CTX-M的季节性ARG副本。 结果表明，未经处理的HWW是ARG的热点，在排入公共下水道之前需要引起注意。

    Hospital wastewaters (HWWs) are reported to be hotspots for antibiotics and antibiotic-resistant bacteria. However, limited information involves the impact of these effluents on dissemination of antibiotic-resistance genes (ARGs). In this study, therefore, seasonally collected HWWs were monitored for overall bacterial load and seven ARGs aadA, tetA, cmlA, sul1, qnrS, ermB and bla CTX-M by using quantitative polymerase chain reaction method. Overall bacterial 16S rRNA copy number was found to be the lowest in winter with 103 copy number/mL, while the highest copy number, with 105 copy number/mL, was observed in both summer and spring. All hospitals tested displayed similar seasonal ARG copy number profile of aadA > tetA > cmlA ≈ sul1 > ermB ≈ qnrS > bla CTX-M. The results indicated that untreated HWWs were hotspots for ARGs and required attention before discharging into public sewer.

    https://link.springer.com/article/10.1007/s00128-020-02792-2