摘要

    抗菌素耐药性（AMR）是一个正在出现的全球性问题。随着滥用和滥用抗生素的消费，抗菌药物耐药的问题日益严重。科学界已明显意识到需要采取监视和监测计划来解决日益严重的AMR传播问题。高通量qPCR技术可有效利用样品/ DNA监测AMR，从而提供成本效益和高通量DNA扩增。该技术已被用于许多研究中，用于分析各种类型样品中的数百种抗药性基因（ARG）。除临床途径外，还广泛报道了在河流，湖泊，废水处理厂，动物粪便，堆肥，土壤等环境样本中进行AMR传播的情况。本章总结了一些利用HT-qPCR技术进行评估的重要研究在世界各地的AMR，详细阐述了在理解各种环境矩阵中AMR复杂动态方面的关键结果和基本特征。

    Antimicrobial resistance (AMR) is an emerging global problem. With the abuse and misuse of antibiotics' consumption, the problem of AMR is increasing. The need for surveillance and monitoring programs for tackling the ever-increasing problem of AMR dissemination has significantly been felt by the scientific community. High-throughput qPCR technology offers cost-effectiveness and high-throughput DNA amplification with efficient use of sample/DNA for monitoring AMR. The technology has already been applied in many studies for analyzing hundreds of antimicrobial-resistance genes (ARGs) in various types of samples. Apart from the clinical routes, AMR dissemination has also widely been reported in environmental samples such as rivers, lakes, wastewater treatment plants, animal manure, composts, soil, etc. This chapter summarizes some of the important studies utilizing HT-qPCR technologies for evaluating AMR in different parts of the world, elaborating the key results and essential features in understanding the complex dynamics of AMR in various environmental matrices.

    https://www.sciencedirect.com/science/article/pii/B9780128188828000140