摘要

    在这项研究中，研究了一个农业扰动的湖泊（即洪湖）及其周围地区的六个抗生素抗性基因（ARG），一个移动遗传元件（int1）及其与微生物群落，抗生素和水质的关系。研究区域中的ARGs和int1在两次采样中每个样本的检测频率均为100％。河流和河口的ARGs高于洪湖。 Sul1是该区域的主要ARG。抗生素，营养素和溶解氧分别与几乎所有ARG呈显着正相关和负相关。这一发现表明减少抗生素和富营养化水平可以降低ARGs的风险。微生物群落的变化对ARG变异有最直接的贡献。但是，考虑到间接影响，环境因素对ARGs的变化贡献了34％，微生物群落贡献了28％，它们的共同作用对ARG的影响贡献了27％。纤毛虫，芽孢杆菌，变形杆菌等的丰富度及其与ARGs的正相关性很显着，表明这些门可能带有ARGs。该研究提供了在典型中国湖泊中ARG分布和扩散的系统概况，以及控制湖泊中这种新兴污染物的新思路。 
Zài zhè xi

    In this study, six antibiotic resistance genes (ARGs), one mobile genetic element (int1), and their relation with microbial communities, antibiotics, and water quality were investigated in and around of an agriculturally disturbed lake, namely, Lake Honghu. The ARGs and int1 in the research area had a 100 % detection frequency in each sample during two sampling times. The ARGs were higher in the rivers and inlets than in Lake Honghu. Sul1 was the main ARG in this area. Antibiotics, nutrients, and dissolved oxygen were significantly, positively, and negatively correlated with nearly all of the ARGs, respectively. This finding suggests that reducing antibiotics and the eutrophication level could reduce the risk of ARGs. Microbial community shift had the most direct contribution to ARG variation. However, when the indirect effect was considered, environmental factors contributed 34 % to the ARGs’ variance, the microbial community contributed 28 %, and their joint effect contributed 27 % to the ARG profiles. The abundance of Firmicutes, Gemmatimonadetes, Proteobacteria, etc. and their positive correlation with ARGs were significant, suggesting that these phyla probably carry ARGs. The study provides a systematic profile of ARG distribution and dissemination in a typical Chinese lake and new ideas to control this emerging contaminant in lakes.

    https://www.sciencedirect.com/science/article/abs/pii/S0304389420304155