摘要

    2017年7月至8月，从中国31个省级行政区域的71个城市（包括28个省会城市，20个地级市和23个县）收集了饮用水样本。此外，还有24个抗生素抗性基因（ARG），通过qPCR对16S rRNA和2个整合酶基因进行定量分析，以研究ARGs的污染程度。结果表明，饮用水中16S的浓度范围为105 – 108拷贝/ 100mL，其处理过程可以有效去除细菌。此外，磺胺类ARGs是中国饮用水中最常见的ARGs，在选定的ARGs中，blaTEM的丰度在所有城市中排名前五位，这表明该基因的污染状况应引起更多关注。 qPCR和相关分析的数据表明intI1在饮用水中ARGs的传播中起着比intI2更重要的作用。此外，不同城市类型之间ARGs的污染程度也没有显着差异。

    Drinking water samples were collected from 71 cities, including 28 provincial capital cities or municipalities, 20 prefecture cities and 23 counties, of 31 provincial-level administrative regions in China from July to August in 2017. Futhermore, 24 Antibiotic Resistance Genes (ARGs), 16S rRNA and 2 integrase genes were quantified by qPCR to investigate the pollution degree of ARGs. The results revealed that the 16S ranged from 105 – 108 copies/100 mL in the drinking water, and its treatment process could effectively remove bacteria. Moreover, sulfonamides-ARGs were the most prevalent ARGs in the drinking water of China, and the abundance of blaTEM ranked top five in all cities among the selected ARGs, indicating that the pollution condition of the genes should be aroused more attention. The data of qPCR and correlation analyses indicated that intI1 played a more crucial role than intI2 in the propagation of ARGs in the drinking water. Additionally, the pollution degree of ARGs among different city types showed no significant difference.

    https://www.sciencedirect.com/science/article/abs/pii/S0147651319311686