摘要

    抗生素抵抗组在世界范围内日益引起人们的关注，特别是考虑将处理过的废水用于填海。本研究选择了废水回收系统（WWRS），该系统由处理系统（TS）和再生系统（RS）组成，并补充了经过处理的废水，并被视为处理和再生的集成系统。高通量qPCR（HT-qPCR）被用于分析WWRS中的283个抗生素抗性基因（ARG）和12个移动遗传元件（MGE）。在WWRS中总共检测到251个ARG和12个MGE亚型。 TS在去除ARG方面表现出良好的性能，最终废水中ARG的数量，相对和绝对丰度大大降低（去除效率为99.07％），这可能归因于生物固体沉降。提高生物固体去除率有助于减少ARGs。在RS中，高质量的流出物显着减少了沿流向下游的ARG的数量和数量。 MGEs在处理后的废水中的减少少于进水（R2 = -0.16，p> 0.05），并且与ARGs表现出紧密的联系。 WWRS中相对丰富的属有：杆菌属，支气管杆菌属，蓝细菌属，蓝杆菌属，不动杆菌属，黄杆菌属和脱氯菌属，它们与ARGs呈显着相关。微生物群落和MGE对ARG的变化贡献了65.64％。这两个因素可能是WWRS中ARG扩散的主要驱动力。因此，在考虑将处理过的废水进行回收时，应注意MGE和丰富的属。

    Antibiotic resistome is a raising concern around the world, especially considering treated wastewater for reclamation. A wastewater reclamation system (WWRS), composed by a treatment system (TS) and a reclaimed system (RS) with supplementation from the treated effluent and considered as an integrated system of treatment and reclamation, was selected in this study. High-throughput qPCR (HT-qPCR) was applied to profile 283 antibiotic resistance genes (ARGs) and 12 mobile genetic elements (MGEs) in the WWRS. A total of 251 ARG and 12 MGE subtypes were detected in the WWRS. The TS exhibited good performance for the removal of ARGs with the number, relative and absolute abundances of ARGs largely decreased (99.07% removal efficiency) in the final effluent, which might be ascribed to biosolid sedimentation. Enhancement of biosolids removal contributed the lessening of ARGs. In the RS, high quality effluent significantly reduced the number and abundance of ARGs along the flow to downstream. MGEs were less reduced in the treated effluent than that of the influent (R2 = −0.16, p > 0.05), and exhibited close connections with ARGs. Arcobacter, Cloacibacterium, Cyanobacteria, Acinetobacter, Flavobacterium and Dechloromonas were the relatively abundant genera in the WWRS, and exhibited significantly correlations with ARGs. Microbial communities and MGEs contributed 65.64% to the changes of ARGs. These two factors may be the main drivers of ARG proliferation in the WWRS. Thus, attention should be paid to MGEs and those abundant genera when considering treated wastewater for reclamation.

    https://www.sciencedirect.com/science/article/abs/pii/S0043135420300774