摘要

    通过使用四种不同的补充材料（小麦秸秆，玉米秸秆，杨木屑和废蘑菇）进行模拟堆肥试验，研究了猪粪堆肥中抗生素抗性基因（ARGs）的变化及其影响因素。结果表明，堆肥后总ARG的相对丰度提高了0.19-1.61个原木，其中tetX，sulI，sulII，dfrA1和aadA是主要贡献者。在所有测试中，整个堆肥过程中ARG分布图和细菌群落的变化均清楚地分为中温-嗜热阶段和冷却-成熟阶段，而不同的补充材料并未产生明显的影响。网络分析表明堆肥堆中ARGs细菌宿主的多样性，多种抗药性细菌的存在以及ARGs与理化因素之间的相关性较弱。值得注意的是，整合子intI1和分枝杆菌（一种潜在的病原体）分别与8个和4个ARG正相关，在堆肥后它们的丰度增加。

    The variation of antibiotic resistance genes (ARGs) and influential factors in pig manure composting were investigated by conducting simulated composting tests using four different supplement materials (wheat straw, corn straw, poplar sawdust and spent mushroom). The results show that the relative abundance of total ARGs increased by 0.19–1.61 logs after composting, and tetX, sulI, sulII, dfrA1 and aadA were the major contributors. The variations of ARG profiles and bacterial communities throughout the composting were clearly divided into mesophilic-thermophilic and cooling-maturation stages in all tests, while different supplement materials did not exert a noticeable influence. Network analysis demonstrated the diversity of bacterial hosts for ARGs, the existence of multiple antibiotic resistant bacteria, and the weak correlations between ARGs and physicochemical factors in the composting piles. Of note, integron intI1 and Mycobacterium (a potential pathogen) were positively correlated with eight and four ARGs, respectively, that displayed increased abundance after composting.

    https://www.sciencedirect.com/science/article/abs/pii/S004565352030374X