摘要

    在这项研究中，通过铁铜替代（mFe / nCu）产生的独特类型的纳米级铜被用于减少后处理猪废水中的ARGs。详细研究了几个关键参数（即理论铜负载量，mFe / nCu用量，空气流速，初始pH和温度）的影响。确定了mFe / nCu系统中纳米级铜对ARGs去除的贡献。此外，通过SEM，TEM和活/死细胞染色检查了mFe / nCu对ARGs的去除机理。结果表明，mFe / nCu工艺可以迅速去除后处理的猪废水中ARG的绝对丰度（约2.4 log）。这种方法的出色性能可能归因于纳米级铜本身的使用，而不是mFe / nCu双金属体系的Fenton样反应引起的氧化。此外，mFe / nCu过程在细菌杀灭和破坏ARGs的结构和功能方面表现出出色的活性，最终可以作为ARGs去除的合理机制。复活实验表明，通过mFe / nCu去除ARGs是永久且不可逆的。总而言之，我们的发现表明mFe / nCu处理可能是从后处理的猪废水中去除ARGs的有前途的先进方法。

    In this study, a distinct type of nanoscale copper, produced by iron-copper replacement (mFe/nCu), was used to reduce ARGs from post-treated swine wastewater. The effects of several key parameters (i. e., theoretical Cu mass loadings, mFe/nCu dosage, air flow rate, initial pH and temperature) were investigated in details. The contribution of nanoscale copper in mFe/nCu system towards ARGs removal was determined. Furthermore, the removal mechanism of ARGs by mFe/nCu was examined by SEM, TEM and living/dead cell staining. Results indicate that the mFe/nCu process can promptly remove the absolute abundances of ARGs (˃2.4 logs) from post-treated swine wastewater. The outstanding performance of this approach could be attributed to the use of nanoscale copper itself rather than the oxidation by Fenton-like reaction of mFe/nCu bimetallic system. Moreover, the mFe/nCu process exhibited an excellent activity in regards bacterial killing and destruction of ARGs structure and function which, ultimately, could serve as a plausible mechanism of ARGs removal. Reactivation experiments showed that ARGs removal by mFe/nCu was permanent and irreversible. Altogether, our findings suggest that mFe/nCu treatment can be a promising advanced method for ARGs removal from post-treated swine wastewater.

    https://www.sciencedirect.com/science/article/abs/pii/S1385894720320817