摘要

    绿茶残留物（GTR）是茶叶生产和加工的副产品，这种类型的农业废物保留了营养成分。这项研究调查了GTR与猪粪的共同堆肥，以及GTR对共同堆肥过程中抗生素抗性基因（ARG）和细菌群落的影响。温度和C / N比表明堆肥在处理后已经成熟。 GTR的添加有效地促进了大多数靶向ARG（tet和sul基因），移动遗传元件（MGE； intI1）和金属抗性基因（MRG； pcoA和tcrB）的丰度降低。冗余分析（RDA）显示，GTR可以通过降低重金属的生物利用度来降低MRG和ARG的丰度。网络分析表明，Firmicutes和放线菌是ARGs的主要宿主，而ARGs，MGEs和MRGs共享相同的潜在宿主细菌。在堆肥过程中添加GTR可能会减少通过水平基因转移（HGT）传播的ARG。 GTR影响细菌群落，从而影响ARG谱图的变化并降低与堆肥产品相关的潜在风险。

    Green tea residues (GTRs) are byproducts of tea production and processing, and this type of agricultural waste retains nutritious components. This study investigated the co-composting of GTRs with swine manure, as well as the effects of GTRs on antibiotic resistance genes (ARGs) and the bacterial community during co-composting. The temperature and C/N ratio indicate compost was mature after processing. The addition of GTRs effectively promoted the reduction in the abundances of most targeted ARGs (tet and sul genes), mobile genetic element (MGE; intI1), and metal resistance genes (MRGs; pcoA and tcrB). Redundancy analysis (RDA) showed that GTRs can reduce the abundance of MRGs and ARGs by reducing the bioavailability of heavy metals. Network analysis shows that Firmicutes and Actinobacteria were the main hosts of ARGs and ARGs, MGEs, and MRGs shared the same potential host bacteria. Adding GTRs during composting may reduce ARGs transmission through horizontal gene transfer (HGT). GTRs affected the bacterial community, thereby influencing the variations in the ARG profiles and reducing the potential risk associated with the compost product.

    https://www.sciencedirect.com/science/article/pii/S0301479720305144