摘要

    受人为活动影响的城市河流可以充当抗生素抗性基因（ARG）的库。这项研究旨在描述尼泊尔城市河流中某些ARG（blaTEM，ermF，mecA和tetA）和1类整合子（intI1）的发生。在一年的时间里，从巴格马蒂河的上游，中游和下游站点定期收集了总共18个水样。除mecA和intl1以外的所有ARG均通过中下游位点的定量聚合酶链反应连续检测到，浓度范围为3.1至7.8 log拷贝/ mL。上游站点的ARG丰度明显较低（p <0.05），反映了人为活动对中下游站点ARGs浓度增加的影响。我们的研究结果表明，尼泊尔城市河水中存在临床相关的ARGs，这表明需要采取缓解策略来防止环境中抗生素耐药性的扩散

    Urban rivers affected by anthropogenic activities can act as reservoirs of antibiotic resistance genes (ARGs). This study aimed to describe the occurrence of selected ARGs (blaTEM, ermF, mecA, and tetA) and a class 1 integron (intI1) in an urban river in Nepal. A total of 18 water samples were collected periodically from upstream, midstream, and downstream sites along the Bagmati River over a 1-year period. All ARGs except mecA and intI1 were consistently detected by a quantitative polymerase chain reaction in the midstream and downstream sites, with concentrations ranging from 3.1 to 7.8 log copies/mL. ARG abundance was significantly lower at the upstream site (p < 0.05), reflecting the impact of anthropogenic activities on increasing concentrations of ARGs at midstream and downstream sites. Our findings demonstrate the presence of clinically relevant ARGs in the urban river water of Nepal, suggesting a need for mitigating strategies to prevent the spread of antibiotic resistance in the environment

    https://www.mdpi.com/2073-4441/12/2/450