摘要

    首先研究了具有集成膜工艺（IMR）的再生水回用系统中抗生素抗性基因（ARGs）的命运。结果表明，在IMR系统中可以有效减少ARGs，1类整合子（intI1）和16S rRNA基因。在IMR系统进行反渗透（RO）过滤后，回用水中所有检测到的ARG的绝对丰度为4.03×104拷贝/ mL，比废水处理的原始进水量低约2-3个数量级。工厂（WWTP）。检测到的基因的最大去除效率高达3.8 log去除值。 IMR系统中所有选定的ARG的总和的日通量急剧下降至（1.02±1.37）×1014拷贝/天，比活性污泥系统（CAS）系统低了1-3个数量级。基于排序分析的强聚类将污水处理厂中的回用水与其他水样分开。网络分析表明存在潜在的抗多种抗生素的细菌。通过微滤和反渗透过滤可以有效地去除潜在的对多种抗生素具有抗药性的细菌，包括梭状芽胞杆菌和地弗洛球菌。这些发现表明，IMR系统可有效去除废水回收系统中的ARGs和潜在的耐多种抗生素的细菌。

    The fate of antibiotic resistance genes (ARGs) in reclaimed water reuse system with integrated membrane process (IMR) was firstly investigated. Results indicated that ARGs, class 1 integrons (intI1) and 16S rRNA gene could be reduced efficiently in the IMR system. The absolute abundance of all detected ARGs in the reuse water after reverse osmosis (RO) filtration of the IMR system was 4.03 × 104 copies/mL, which was about 2–3 orders of magnitude lower than that in the raw influent of the wastewater treatment plants (WWTPs). Maximum removal efficiency of the detected genes was up to 3.8 log removal values. Daily flux of the summation of all selected ARGs in the IMR system decreased sharply to (1.02 ± 1.37) ×1014 copies/day, which was 1–3 orders of magnitude lower than that in the activated sludge system (CAS) system. The strong clustering based on ordination analysis separated the reuse water from other water samples in the WWTPs. Network analysis revealed the existence of potential multi-antibiotic resistant bacteria. The potential multi-antibiotic resistant bacteria, including Clostridium and Defluviicoccus, could be removed effectively by microfiltration and RO filtration. These findings suggested that the IMR system was efficient to remove ARGs and potential multi-antibiotic resistant bacteria in the wastewater reclamation system.

    https://www.sciencedirect.com/science/article/abs/pii/S0304389419309793