摘要

    蓝藻水华引起的抗生素抗性基因（ARG）和微囊藻毒素（MC）的二次污染问题已成为全球性的重大问题。这两种污染物共存于饮用水处理厂（DWTP）中，但它们之间的确切关系需要进一步阐明。在这里，首先使用高通量定量实时PCR和酶联免疫吸附试验来研究ARGs和MC在实际DWTP中的行为。经过现场调查，在实验室条件下研究了MC对ARGs水平转移的影响，并在细胞和分子水平上探索了机理。 MC可以促进ARG的传播，特别是在相对稳定和稳定的环境（例如生物膜）中。 MC-LR是最有效的微囊藻毒素亚型，促进了共轭转移，比对照组高25.13倍。 MC通过调节一系列参与共轭转移的基因系统，刺激活性氧（ROS）的形成并增加细胞膜通透性，影响了ARGs的水平转移。本研究可为控制饮水厂污水处理厂的ARGs和MCs提供理论依据，对于科学评价饮用水安全具有重要意义。

    Problems with antibiotic resistance genes (ARGs) and secondary pollution from microcystins (MCs), caused by cyanobacterial blooms have become significant global issues. These two pollutants co-occur in drinking water treatment plants (DWTPs), but the exact relationships between them requires further clarification. Here, a high-throughput quantitative real-time PCR and enzyme-linked immunosorbent assay were used to investigate the behavior of ARGs and MCs in a practical DWTP in the first place. After the on-site investigation, the effect of MCs on the horizontal transfer of ARGs was studied under laboratory conditions, and mechanisms explored at both cellular and molecular levels. MCs could promote the spread of ARGs, especially in relatively stationary and stable environments such as biofilms. MC-LR was the most efficient microcystin subtype promoting conjugative transfer, which was 25.13 times higher than for the control group. MCs affected the horizontal transfer of ARGs by regulating a series of gene systems involved in conjugative transfer, stimulating the formation of reactive oxygen species (ROS), and increasing cell membrane permeability. This study can provide a theoretical basis for the control of ARGs and MCs in DWTPs, which is of great significance for the scientific assessment of drinking water safety.

    https://www.sciencedirect.com/science/article/abs/pii/S0269749119346391