摘要

    研究了复合微生物剂对猪粪堆肥中抗生素抗性基因（ARGs）的影响。结果表明，在重复剂量组和高剂量组中，添加微生物剂可促进总ARG的降低，并且绝对丰度（77.2％）和相对丰度（64.5％）的最大去除效率分别得到观察。随着Firmicutes和Tn916 / 1545的减少，四类ARGs减少，而随着intI1和堆肥中各种潜在宿主的扩散，两类ARGs增加。各组的堆肥阶段（中温-嗜热和冷却-成熟阶段）决定了ARG的概况和细菌群落。但是，添加微生物剂会加速堆肥阶段的变化，并可能改变潜在的ARG宿主，从而影响ARG的去除。值得注意的是，intl1和两种潜在的病原体（分枝杆菌和芽孢杆菌）与几种增加的ARGs正相关，这暗示了堆肥产品的潜在风险。 

    The influence of compound microbial agents on antibiotic resistance genes (ARGs) in pig manure composting was investigated. The results show that the addition of microbial agents promoted the reduction of total ARGs and the maximum removal efficiencies for absolute abundance (77.2%) and relative abundance (64.5%) were observed in the repeated dose and high dose groups, respectively. Four categories of ARGs declined with the reduction of Firmicutes and Tn916/1545, whereas two categories increased with the proliferation of intI1 and various potential hosts in the composting. The ARG profiles and bacterial communities were shaped by composting stages (mesophilic-thermophilic and cooling-maturation stages) in all groups. However, the addition of microbial agents accelerated the variation of composting stages, and may change the potential ARG hosts which influences the removal of ARGs. Of note, intI1 and two potential pathogens (Mycobacterium and Bacillus) correlated positively to several increased ARGs, implying the possible risks of compost products.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420312384