摘要

本研究探讨了的猪肥对小麦秸秆不同质量比例（3：7,5：5和7：3，即对照反应器C1，C2和C3）对抗生素抗性基因（ARGs）变异和微生物群落厌氧消化（AD）的影响。 C1，C2和C3的累积沼气产量分别为1711,3857和3226mL。 在AD后，ARG的总相对丰度在C3中减少了4.23个对数，而在C1和C2中减少量分别仅为1.03个对数和1.37个对数。 网络分析显示，Solibacillus，Enterococcus，Facklamia，Corynebacterium 1和Acinetobacter是ermB，sul1和dfrA7的潜在宿主。 冗余分析表明，细菌群落和环境因素在ARG的变异中起着重要的作用。 因此，在重复使用AD处理的动物粪便之前，应考虑降低ARG含量。

This study explored the effects of different mass ratios of swine manure relative to wheat straw (3:7, 5:5, and 7:3, i.e., control reactors C1, C2, and C3, respectively) on variations in antibiotic resistance genes (ARGs) and the microbial community during anaerobic digestion (AD). The cumulative biogas production volumes were 1711, 3857, and 3226 mL in C1, C2, and C3, respectively. After AD, the total relative abundance of ARGs decreased by 4.23 logs in C3, whereas the reductions were only 1.03 and 1.37 logs in C1 and C2, respectively. Network analysis showed that the genera Solibacillus, Enterococcus, Facklamia, Corynebacterium_1, andAcinetobacter were potential hosts of ermB, sul1, and dfrA7. Redundancy analysis showed that the bacterial communities and environmental factors played important roles in the variation in ARGs. Thus, reductions in ARGs should be considered before reusing animal manure treated by AD.

https://www.sciencedirect.com/science/article/pii/S0960852417300913