摘要

    猪废水中的抗生素和抗生素抗性基因（ARGs）对周围的水和土壤生态系统产生不可逆转的影响。在此，构建集成垂直流人工湿地 (IVCW) 以评估沸石和植物对磺胺 (SM)、四环素 (TC) 和相关 ARG (tetW、tetO、tetM、sul I、sul II) 去除的影响, 和 sul III) 来自消化的猪废水。还研究了微生物群落结构。结果表明，具有沸石基质和植物系统 (ZP) 的 IVCW 对 N、抗生素和 ARG 的去除率分别为 97.9%、95.0% 和 95.1%。此外，沸石体系对 SM 的吸附较高，对 TC 的吸附较低。 ZP 系统中抗生素的较高去除率可能是由于沸石中假单胞菌、不动杆菌和芽孢杆菌的富集增强了微生物降解。此外，芦竹对抗生素去除的影响有限，不利于 ARGs 的去除。 sul(I)、sul(II)、sul(III)、tet(M)和tet(O)的绝对丰度与16S rDNA的绝对丰度显着正相关。然而，没有发现抗生素浓度与流出物中相关 ARG 的丰度之间存在显着相关性。

    Antibiotics and antibiotic resistance genes (ARGs) in swine wastewater have an irreversible impact on the surrounding water and soil ecosystems. Herein, integrated vertical-flow constructed wetlands (IVCWs) were constructed to assess the effects of zeolite and plants on the removal of sulfonamides (SMs), tetracyclines (TCs), and related ARGs (tetW, tetO, tetM, sul I, sul II, and sul III) from digested swine wastewater. The microorganism community structure was also investigated. Results showed that IVCWs with a zeolite substrate and plant system (ZP) exhibited a favorable removal performance for N, antibiotics, and ARGs at 97.9%, 95.0%, and 95.1%, respectively. Moreover, zeolite systems showed higher adsorption of SMs, lower adsorption of TCs. The higher removal rate of antibiotics in ZP systems might be due to the enhanced microbial degradation with the enrichment of Pseudomonas, Acinetobacter, and Bacillus in zeolite. Furthermore, Arundo donax had limited impact on antibiotics removal and was not conducive to the removal of ARGs. The absolute abundances of sul(I), sul(II), sul(III), tet(M), and tet(O) were significantly positively correlated with the absolute abundance of 16S rDNA. However, no significant correlation was found between the concentration of antibiotics and the abundance of related ARGs in the effluent.

   https://www.sciencedirect.com/science/article/abs/pii/S0048969720312766