摘要

    生物炭因其环境影响而受到越来越多的关注，这在废水处理中很有前景。在本研究中，评估了 β-环糊精功能化生物炭 (β-BC) 在重金属和染料共同胁迫下去除废水处理中抗生素抗性基因 (ARG) 的性能。结果表明，当每天进料进水中存在 20 mg/L 重金属 (HMs) 和 150 mg/L 甲基橙 (MO) 时，只有 0.05 mg/L HMs 残留和 96.79%–98.84% MO 去除效率在 β- BC 添加剂组，分别与对照组的 0.16 mg/L 和 87.92%–94.11% 相比，表明 β-BC 有利于污染物去除的性能。为了评估 β-BC 在多污染物胁迫系统中对 ARG 的作用，tet W、tet M、sul-1、sul-2、blaTEM、oxa-1、qnr-S、erm-B 和 intI-1 是确定。与没有 β-BC 添加剂的相应组相比，当 β-BC 存在时，所有鉴定的 ARG 的相对丰度都降低了。探索了微生物群落的多样性和组成，并推测潜在耐药细菌的减少是去除 ARG 的驱动因素。总之，我们的研究表明，β-BC 在 HMs-MO 共污染物下的连续废水处理过程中具有促进 ARGs 去除的能力。

    Biochar has been received increasing concerns regarding its environmental effect, which is promising in wastewater treatment. In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated. Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%–98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%–94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal. To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified. The relative abundance of all identified ARGs are decreased when β-BC presence compared to the corresponding groups without β-BC additive. The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal. In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.

    https://www.sciencedirect.com/science/article/abs/pii/S0048969720315047