发布者：抗性基因网 时间：2018-02-25 浏览量：1521
铜是猪粪中含量最丰富的重金属之一。以铜抗性基因[铜抗性基因( CRGs ) : pcoA、cusA、copA和CRB]、抗生素抗性基因[抗生素抗性基因( ARGs ) : erm ( A )和erm ( B )]和intl1为研究对象，研究了Cu在三个水平下（0 mg kg - 1、200 mg kg - 1和2000 mg kg - 1，即对照，Cu200和Cu2000处理）对实验室规模的好氧堆肥系统的影响。结果表明，猪粪堆肥后，pcoA、tcrB、erm ( A )、erm ( B )和intl10的绝对丰度降低，而copA和cusA的绝对丰度增加。冗余分析表明，温度显著影响CRGs、ARGs和intl 1的变化。CRGs、ARGs和int1的下降与交换性铜水平呈正相关。在不同处理下，细菌群落可根据堆肥时间进行分组，其中高浓度铜对细菌群落的具有更持久影响。网络分析表明，CRGs、ARG和int1以及细菌群落的共同发生是导致CRGs、ARG和int1变化的主要因素。因此，温度、铜和细菌群落组成的变化对添加铜堆肥过程中CRGs、ARGs和intl 1的变化有重要影响。
Copper is one of the most abundant heavy metals present in swine manure. In this study, a laboratory-scale aerobic composting system was amended with Cu at three levels (0, 200, and 2000 mg kg-1, i.e., control, Cu200, and Cu2000 treatments, respectively) to determine its effect on the fate of copper resistance genes [copper resistance genes (CRGs): pcoA, cusA, copA, andtcrB], antibiotic resistance genes [antibiotic resistance genes (ARGs): erm(A) and erm(B)], andintl1. The results showed that the absolute abundances of pcoA, tcrB, erm(A), erm(B), and intl1were reduced, whereas those of copA and cusA increased after swine manure composting. Redundancy analysis showed that temperature significantly affected the variations in CRGs, ARGs, and intl1. The decreases in CRGs, ARGs, and intI1 were positively correlated with the exchangeable Cu levels. The bacterial community could be grouped according to the composting time under different treatments, where the high concentration of copper had a more persistent effect on the bacterial community. Network analysis determined that the co-occurrence of CRGs, ARGs, and intI1, and the bacterial community were the main contributors to the changes in CRGs, ARG, and intl1. Thus, temperature, copper, and changes in the bacterial community composition had important effects on the variations in CRGs, ARGs, and intl1 during manure composting in the presence of added copper.
https://www.frontiersin.org/articles/10.3389/fmicb.2017.00344/full