摘要

  由于其最终产品可用作土壤营养改良剂，因此越来越多地采用污泥堆肥。 尽管 C/N 比在堆肥的质量和过程中很重要，但关于营养能量（碳和氮）对污泥堆肥过程中抗生素抗性基因（ARGs）命运的影响的信息很少。 本研究调查了污泥堆肥过程中 ARGs、微生物群落和功能特征的动态变化。 开发了三个水平的碳氮（20:1、25:1 和 30:1），用于用发酵秸秆堆肥污泥以及仅是污水污泥的对照（C/N = 9.5:1）。 这项工作的一个新发现是最高的初始 C/N 比（30:1）可以延长嗜热期，这有助于减少一些目标 ARG。 一些ARGs（sul1、sul2和aadA1）与多种代谢途径呈负相关，难以去除。

  Sludge composting is increasingly adopted due to its end product for application as a soil nourishment amendment. Although the ratio of C/N is significant in the quality and process of composting, little information has been obtained from the effects of nutritional energy (carbon and nitrogen) on the fate of antibiotic resistance genes (ARGs) during sludge composting. Dynamic variations of ARGs, microbial community as well as functional characteristics during composting of sludge were investigated in this study. Three levels of carbon to nitrogen (20:1, 25:1 and 30:1) were developed for the composting of sludge with fermented straw plus a control which was just sewage sludge (C/N = 9.5:1). A novel finding of this work is that the highest initial C/N ratio (30:1) could prolong the thermophilic period, which was helpful to reduce some target ARGs. Some ARGs (sul1, sul2, and aadA1) had negative correlation with multiple metabolic pathways, which were difficult to remove.

  https://www.sciencedirect.com/science/article/abs/pii/S0960852419317432