摘要

 这项研究检查了来自一个大型淡水池塘养殖系统的水和沉积物中抗生素抗性基因 (ARG) 的概况。 qPCR 阵列用于量化 ARG（16S、Tetx、Tetw、TetG、Intll 和 Sull），并通过 16S rRNA 基因测序分析微生物群落结构。 检测到大量 ARG（2 8 8）。 沉积物的 ARG 丰富度显着高于水，平均检测到 15 个以上的基因（p < 0.01）。 沉积物样品显示出显着更高的分类多样性和更高的 Gammaproteobacteria、Betaproteobacteria 和 Flavobacteria 丰度。 在抗生素抗性基因和育种期之间观察到显着相关性。 池塘样本的分类多样性显着高于沟渠样本（p < 0.05），表明池塘养殖系统可以作为本地水库将 ARGs 传播到农村社区的水生环境中。

  This study examined the profiles of antibiotic resistance genes (ARGs) in water and sediments from one large-scale freshwater pond farming system. A qPCR array was used to quantify ARGs (16S, Tetx, Tetw, TetG, Intll, and Sull) and microbial community structure was analyzed by 16S rRNA gene sequencing. A large number of ARGs (2 8 8) were detected. The ARG richness of the sediments was significantly higher than that of water and an average of 15 more genes were detected (p < 0.01). Sediment samples showed significantly higher taxonomic diversity and higher abundance of Gammaproteobacteria, Betaproteobacteria, and Flavobacteria. A significant correlation was observed between antibiotic resistance genes and breeding periods. The taxonomic diversity of the samples in ponds was significantly higher than that in ditch samples (p < 0.05), suggesting that pond farming systems could act as a local reservoir to spread ARGs into aquatic environments of rural communities.

   https://www.sciencedirect.com/science/article/abs/pii/S0048969719346170