摘要
通过比较原始和人为影响的环境，宏基因组分析可鉴定抗生素抗性基因（ARG）和移动遗传因子（MGE）。 我们收集了来自3个不同环境的样品：受人为活动影响很小的冰川土壤，年龄为5821 BP（人类抗生素之前）的深层永久冻土和珠江沉积物。 磺胺类药物，四环素类药物和氟喹诺酮类药物在沉积物样品中很常见。 冻土中未发现磺胺类药物和四环素类药物; 冰川土壤中也没有发现四环素。 沉积物中的ARGs比冰川土壤和永久冻土中的ARGs更丰富多样。 沉积物中还存在更多类型的阻力机制。 MGEs的多样性与ARG的丰度和多样性显着相关。 结果将有助于未来工作人员更好地了解ARGs在受人为活动影响程度不同的环境中的分布情况。
Antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) can be identified with metagenomic analyses comparing relatively pristine and human-impacted environments. We collected samples from 3 different environments: glacial soil little affected by anthropogenic activity, deep permafrost dated to 5821 BP (before human antibiotics), and sediment from the Pearl River. Sulfonamides, tetracyclines, and fluoroquinolones were common in the sediment samples. Sulfonamides and tetracycline were not found in permafrost; tetracycline was also not found in glacial soil. ARGs from the sediment were more abundant and diverse than those from glacial soil and permafrost. More types of resistance mechanisms were also present in the sediment. The diversity of MGEs was significantly correlated with the abundance and diversity of ARGs. The result will help future workers to better understand the distribution of ARGs among environments more or less impacted by anthropogenic activities.
https://www.sciencedirect.com/science/article/pii/S0269749117333730
