摘要

       研究了通过使用由底物和床隔间组成的新型蠕虫反应器，从混合的过量活性污泥 (EAS) 中消除抗生素抗性基因 (ARG)，以有效处理不同的水果和蔬菜废物 (FVW)。 ARG（tet G、tet M 和 sul 1）和移动遗传元件基因（intl 1）是靶向的，通过对它们在隔间和新鲜蚯蚓中的丰度进行定量分析，证实了底物隔间的显着减少 即使不同类型的 FVW 降低程度不同，也可以单独添加 EAS 和 EAS 对 FVW 进行处理。 在最终产品积累的床隔间中没有发现明显的减少。 对于新鲜铸件，ARGs 和 intl 1 相对于总细菌 16S rDNA 的相对丰度显着下降。 本研究为在 FVW 和 EAS 的蚯蚓堆肥过程中正确控制 ARGs 提供了见解。

       Elimination of antibiotic resistance genes (ARGs) from excess activated sludge (EAS) mixed for effective treatment of different fruit and vegetable waste (FVW) by using a novel vermireactor consisted of substrate and bed compartments was investigated. ARGs (tet G, tet M and sul 1) and mobile genetic element gene (intl 1) were targeted and, through quantitative analysis of their abundances in both the compartments and the fresh cast of earthworms, significant reductions in substrate compartments were confirmed for the treatments for FVW added with EAS and EAS alone even if the reduction extents differed among the types of FVW. Apparent reductions were not found in the bed compartment where the final products accumulated. For the fresh cast, the relative abundances of ARGs and intl 1 against to the total bacterial 16S rDNA decreased markedly. The present study provided an insight for proper controlling of ARGs during vermicomposting of FVW and EAS. 

https://www.sciencedirect.com/science/article/abs/pii/S096085242100033X