摘要

       这项工作旨在研究生物炭对猪粪（SM）厌氧消化（AD）过程中产甲烷和抗生素抗性基因（ARGs）命运的影响。在 5-10% 的最佳生物炭用量下，甲烷产率显着提高了 25%，这归因于种间直接电子转移（DIET）的增强。添加 Biochar 减轻了对细胞色素-c 作为种间电子连接组件的需求，并丰富了 DIET 中涉及的微生物。 Defluviitoga、Thermovirga和Cloacibacillus在生物炭添加中占优势，可能与Methanothrix一起参与DIET。在 biochar 处理中，parC、tetX、blaCTX-M、blaTEM、aac(6')-Ib-cr、ermB 和 tetW 的丰度显着降低，并且在 B-5% 中的 intI1 去除量比对照增加了 15%。结构方程模型表明 intI1 (λ = 0.59, P < 0.001) 对 ARGs 具有最标准化的直接影响，而 biochar 通过改变 intI1 (λ = 0.41, P < 0.01) 和微生物结构 (λ = -0.24, P < 0.05)。

       This work aimed to study the effects of biochar on methanogenesis and antibiotic resistance genes (ARGs) fate during anaerobic digestion (AD) of swine manure (SM) was investigated. Under the optimal biochar dosage of 5–10%, methane yield was significantly improved by 25%, ascribing to the enhancement of direct interspecies electron transfer (DIET). Biochar addition alleviated the need of cytochrome-c as interspecies electron connection components and enriched the microbes involved in DIET. Defluviitoga, Thermovirga and Cloacibacillus were dominant with biochar addition and might participate in DIET together with Methanothrix. The abundance of parC, tetX, blaCTX-M, blaTEM, aac(6′)-Ib-cr, ermB and tetW were significantly reduced in biochar treatments and intI1 removal increased by 15% in B-5% than the control. Structural equation models demonstrated that intI1 (λ = 0.59, P < 0.001) had the most standardized direct effects on ARGs, while biochar indirectly affected ARGs by changing intI1 (λ = 0.41, P < 0.01) and microbial structure (λ = −0.24, P < 0.05).

https://www.sciencedirect.com/science/article/abs/pii/S0960852421000171