摘要

       粪便储存设施是重要的控制点，可在猪粪浆被土地施用之前减少猪粪浆中的抗生素抗性基因 (ARG)。然而，关于进入粪便储存设施的外源性化学物质如何影响 ARGs 的命运，我们知之甚少。本研究的目的是分析六种常用的坑添加剂和四种设施消毒剂对猪粪浆中 ARGs 浓度的影响。实验室规模的反应器，每个含有大约 50 升液体猪粪，加入添加剂或消毒剂，并取样 40 天。监测了七个抗生素抗性基因以及 intI1 基因和 16S rRNA 基因。在测试的六种添加剂中，与无添加剂对照相比，Sludge Away 显着降低了 erm(C)、erm(F)、tet(Q) 和 16S rRNA 基因的时间平均绝对丰度。在测试的四种消毒剂中，与未使用的消毒剂相比，Tek-Trol 显着降低了 erm(B)、erm(C)、erm(F)、intI1、tet(Q) 和 tet(X) 的时间平均绝对丰度- 消毒控制。根据 Spearman 的秩相关，erm(F)、tet(Q) 和 tet(X) 三个基因表现出强到完全正相关，两个基因 erm(B) 和 tet(O) 在添加剂和消毒剂测试。总体而言，消毒剂在控制 ARG 的绝对丰度方面比坑添加剂更有效。

       Manure storage facilities are critical control points to reduce antibiotic resistance genes (ARGs) in swine manure slurry before the slurry is land applied. However, little is known about how exogenous chemicals entering the manure storage facilities may affect the fate of ARGs. The objective of this study was to analyze the impact of six commonly used pit additives and four facility disinfectants on the concentration of ARGs in swine manure slurry. Bench scale reactors, each containing approximately 50 L of liquid swine manure, were dosed with additives or disinfectants and were sampled for 40 days. Seven antibiotic resistance genes along with the intI1 gene and the 16S rRNA gene were monitored. Out of the six additives tested, Sludge Away significantly reduced the time-averaged absolute abundance of erm(C), erm(F), tet(Q), and the 16S rRNA gene as compared to the no additive control. Out of the four disinfectants tested, Tek-Trol significantly reduced the time-averaged absolute abundance of erm(B), erm(C), erm(F), intI1, tet(Q), and tet(X) than did the no-disinfectant control. According to Spearman's rank correlation, three genes erm(F), tet(Q), and tet(X) showed a strong to perfectly positive correlation and the two genes erm(B) and tet(O) showed a moderate to strong correlation in both the additive and disinfectant tests. Overall, the disinfectants were more effective in controlling the absolute abundance of ARGs than were the pit additives.

https://www.sciencedirect.com/science/article/abs/pii/S0048969720368182