摘要

       本研究采用高通量定量聚合酶链反应方法，综合评估农业土地利用变化对太湖流域降雨后农业径流抗生素耐药性的影响。我们的研究结果首次在该地区显示果园径流比传统农田径流含有更多种类和丰富的抗生素抗性基因 (ARG)。网络分析表明，果园径流通过水平基因转移具有很强的 ARG 传播能力。这些结果表明，居民可能面临比以前更高的公共卫生威胁。此外，本研究证实水稻-小麦轮作系统在调节土壤抗生素抗性谱中起着关键作用。本研究利用16S rRNA高通量测序技术，阐明了抗生素耐药组与土壤微生物组组成之间的关系。最后，我们讨论了驱动土壤抗生素抗性组变化的关键环境因素。总之，本研究深入了解了环境 ARGs 向生活在太湖流域的人们的传播。

       In this study, we use high-throughput quantitative polymerase chain reaction approaches to comprehensively assess the effects of agricultural land-use change on the antibiotic resistome of agricultural runoffs after rainfalls in Lake Tai Basin. For the first time in this region, our findings show that orchard runoffs harbored more diverse and abundant antibiotic resistance genes (ARGs) than traditional cropland runoffs. Network analysis demonstrated that orchard runoffs possessed a strong ability for ARG dissemination via horizontal gene transfer. These results suggest that residents might be exposed to a higher public health threat than before. Moreover, the present study confirmed that the rice–wheat rotation system plays a key role in regulating the soil antibiotic resistome profile. Using 16S rRNA high-throughput sequencing technology, this study clarified the relationships between the antibiotic resistome and soil microbiome composition. Finally, we discuss the key environmental factors driving changes in the soil antibiotic resistome. In summary, this study gives insight into the dissemination of environmental ARGs to the people living in the Lake Tai Basin.

https://www.sciencedirect.com/science/article/abs/pii/S0048969720383819